β-Actin (D6A8) Rabbit mAb?(HRP Conjugate)

• 产品编号：CST-12620S      品牌：CST       原厂货号：12620S
• 产品分类：抗体 > 一抗 > 酶标记一抗 > 辣根过氧化物酶（HRP）
• 应用分类：

描述：

β-Actin (D6A8) Rabbit mAb（偶联HRP）可以识别内源性总的β-Actin 蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人β-actin蛋白的氨基端一致。Cell Signaling Technology抗体与辣根过氧化物酶（HRP）的碳水化合物基团偶联。HRP偶联的抗体被认为与未偶联的β-Actin (D6A8) Rabbit mAb #8457具有相同的交叉反应性。Actin，一种广泛表达的真核蛋白，是细胞骨架的重要组分。哺乳动物细胞中发现了至少6种亚型。非肌肉β- 和γ-actin，也被称为细胞质actin，主要表的在非肌肉细胞中，扩至细胞结构和运动（1）。α-cardiac和α-skeletalactin分别在心脏条纹肌和骨骼肌中，两种平滑肌actin，α- 和γ-actin，分别主要在血管平滑肌和肠平滑肌中表达。这些actin亚型会调控肌肉细胞的收缩力（1）。Actin主要作为一种纤维聚合物存在，F-actin。在多个过程中应激细胞骨架重组信号，包括胞质分裂，内吞或压力，cofilin促进F-actin的断裂和解聚，导致单球形的形式，G-actin增加（2）。Arp2/3复合物会促进F-actin断裂，以形成新的actin（2）。研究证明在原始乳腺癌细胞中actin是高度磷酸化的（3）。研究也发现凋亡条件下体外和心脏肌肉以及骨骼肌中都能观察到actin的清除（4-6）。Caspase-3对actin的清除会加速泛素/蛋白酶体依赖的肌肉蛋白酶解（6）。

1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
3. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
4. Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
5. Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
6. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Hamster, Chicken, Pig

Specificity / Sensitivity

β-Actin (D6A8) Rabbit mAb (HRP Conjugate) recognizes endogenous levels of total β-actin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human β-actin protein.

Description

This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated antibody β-Actin (D6A8) Rabbit mAb #8457.

Background

Actin, a ubiquitous eukaryotic protein, is the major component of the cytoskeleton. At least six isoforms are known in mammals. Nonmuscle β- and γ-actin, also known as cytoplasmic actin, are predominantly expressed in nonmuscle cells, controlling cell structure and motility (1). α-cardiac and α-skeletal actin are expressed in striated cardiac and skeletal muscles, respectively; two smooth muscle actins, α- and γ-actin, are found primarily in vascular smooth muscle and enteric smooth muscle, respectively. These actin isoforms regulate the contractile potential of muscle cells (1). Actin exists mainly as a fibrous polymer, F-actin. In response to cytoskeletal reorganizing signals during processes such as cytokinesis, endocytosis, or stress, cofilin promotes fragmentation and depolymerization of F-actin, resulting in an increase in the monomeric globular form, G-actin (2). The ARP2/3 complex stabilizes F-actin fragments and promotes formation of new actin filaments (2). Research studies have shown that actin is hyperphosphorylated in primary breast tumors (3). Cleavage of actin under apoptotic conditions has been observed in vitro and in cardiac and skeletal muscle, as shown in research studies (4-6). Actin cleavage by caspase-3 may accelerate ubiquitin/proteasome-dependent muscle proteolysis (6).

1. Herman, I.M. (1993) Curr. Opin. Cell Biol. 5, 48-55.
2. Condeelis, J. (2001) Trends Cell Biol. 11, 288-293.
3. Lim, Y.P. et al. (2004) Clin. Cancer Res. 10, 3980-3987.
4. Kayalar, C. et al. (1996) Proc. Natl. Acad. Sci. USA. 93, 2234-2238.
5. Communal, C. et al. (2002) Proc. Natl. Acad. Sci. USA. 99, 6252-6256.
6. Du, J. et al. (2004) J. Clin. Invest. 113, 115-123.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibody.