Smad2 (D43B4) XP??Rabbit mAb?(Biotinylated)

• 产品编号：CST-12584S      品牌：CST       原厂货号：12584S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

Smad2 (D43B4) XP^® Rabbit mAb（生物素偶联）可以识别内源性的总Smad2 蛋白。此抗体与Smad3蛋白无交叉反应。此单克隆抗体由合成肽段免疫动物产生，该肽段与人Smad2蛋白邻近氨基末端的氨基酸残基序列一致。此CST抗体在优化的条件下与生物素偶联。偶联了生物素的抗体与未偶联的Smad2 (D43B4)XP^® Rabbit mAb #5339有相同的反应性。Smad信号转导分子家族成员是胞内通路中TGF-β信号从细胞表面传送到细胞核的重要组成部分。Smad 可被分为三类：受体调控型Smad（简称R-Smad），主要包括Smad1,2,3,5和8；共同介导型Smad（co-Smad）包括Smad4；还有拮抗型或称抑制型Smad（I-Smads），包括Smad6和Smad7[1-5]。激活的I型受体与特异的R-Smad有关，并能在R-Smad的保守的C端SSXS基序处对其磷酸化。磷酸化的R-Smad与受体分离，再与co-Smad（Smad4）形成异侧复合物，使复合物迁移入核。一旦进入核内，Smad可以各种DNA结合蛋白为目标调控转录反应。[6-8]

1. Heldin, C.H. et al. (1997) Nature 390, 465-71.
2. Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.
3. Derynck, R. et al. (1998) Cell 95, 737-40.
4. Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
5. Whitman, M. (1998) Genes Dev 12, 2445-62.
6. Wu, G. et al. (2000) Science 287, 92-7.
7. Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
8. Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.

原厂资料：

Specificity / Sensitivity

Smad2 (D43B4) XP^® Rabbit mAb (Biotinylated) recognizes endogenous levels of total Smad2 protein. This antibody does not cross-react with Smad3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of mouse Smad2 protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Smad2 (D43B4) XP^® Rabbit mAb #5339.

Background

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-β signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7 (1-5). Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses (6-8).

1. Heldin, C.H. et al. (1997) Nature 390, 465-71.
2. Attisano, L. and Wrana, J.L. (1998) Curr Opin Cell Biol 10, 188-94.
3. Derynck, R. et al. (1998) Cell 95, 737-40.
4. Massagué, J. (1998) Annu Rev Biochem 67, 753-91.
5. Whitman, M. (1998) Genes Dev 12, 2445-62.
6. Wu, G. et al. (2000) Science 287, 92-7.
7. Attisano, L. and Wrana, J.L. (2002) Science 296, 1646-7.
8. Moustakas, A. et al. (2001) J Cell Sci 114, 4359-69.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibody.