CBARA1/MICU1 (D4P8Q) Rabbit mAb

• 产品编号：CST-12524S      品牌：CST       原厂货号：12524S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CBARA1/MICU1 (D4P8Q) Rabbit mAb兔单抗识别内源性的总CBARA1/MICU1蛋白。该单克隆抗体通过用合成肽免疫动物制备，该合成肽与人CBARA1/MICU1蛋白赖氨酸（110位）附近的残基一样。CBARA1/MICU1是一个与线粒体内膜相关的线粒体蛋白，由两个EF-手形螺旋-环-螺旋基序组成。CBARA1/MICU1参与线粒体的钙进入、代谢耦联的胞浆钙离子瞬变和基质脱氢酶的激活（1）。线粒体CBARA1/MICU1通过与单向转运体孔隙亚基MCU相互作用，在保持正常的线粒体钙浓度低于平衡水平中是需要的（2）。CBARA1/MICU1在胰腺β-细胞线粒体摄取和维持胰岛素分泌中是重要的（3）

1. Perocchi, F. et al. (2010) Nature 467, 291-6.
2. Mallilankaraman, K. et al. (2012) Cell 151, 630-44.
3. Alam, M.R. et al. (2012) J Biol Chem 287, 34445-54.

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原厂资料：

Specificity / Sensitivity

CBARA1/MICU1 (D4P8Q) Rabbit mAb recognizes endogenous levels of total CBARA1/MICU1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys110 of human CBARA1/MICU1 protein.

Background

CBARA1/MICU1 is a mitochondrial protein associated to the mitochondrial inner membrane that is comprised of two EF hand helix-loop-helix motifs. CBARA1/MICU1 is involved in mitochondrial calcium entry, metabolic coupling between cytosolic calcium transients, and activation of matrix dehydrogenases (1). Mitochondrial CBARA1/MICU1 is required to preserve normal mitochondrial calcium concentration below the equilibrium level by interacting with the uniporter pore-forming subunit MCU (2). CBARA1/MICU1 is important in pancreatic β-cell mitochondrial calcium uptake and sustained insulin secretion (3).

1. Perocchi, F. et al. (2010) Nature 467, 291-6.
2. Mallilankaraman, K. et al. (2012) Cell 151, 630-44.
3. Alam, M.R. et al. (2012) J Biol Chem 287, 34445-54.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.