Eps15 (D3K8R) Rabbit mAb

• 产品编号：CST-12460S      品牌：CST       原厂货号：12460S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Eps15 (D3K8R) Rabbit mAb兔单抗能够检测内源性的Eps15总蛋白。 基于序列比对分析，该抗体可能会与 Eps15a 和Eps15b发生交叉反应。但不会与 Eps15R发生交叉反应。该单克隆抗体是经由合成的围绕人 Eps15 蛋白 Pro630的氨基酸肽段免疫动物而生产的。Eps15（EGFR通路底物15）首先被发现作为EGFR激酶活性的底物（1）。Eps15有三部分结构，氨基末端部分包含三个进化上保守的EH蛋白-蛋白相互作用区域，中央部分推测的卷曲螺旋区域，为组成性寡聚化所需，羧基末端区域包含多个天冬氨酸-脯氨酸-苯丙氨酸三联重复，组成AP2结合域。羧基末端也包含两个泛素相互作用基序（UIM），其中后一个为Eps15与泛素结合所不可缺少的（1）。若干证据支持Eps15在clathrin介导的内吞中发挥作用，包括突出囊泡的内吞。Eps15像结合其它涉及内吞或突触囊泡回收蛋白如synaptojanin1和epsin一样结合到AP-2。另外，Eps15与质膜clathrin包被的小凹和囊泡标志物共定位（2）。的确，Eps15被显示出调节内体的c-Met（3）和EGFR（4）转运，可能是通过其UIM与泛素标签相互作用招募这些泛素化的受体到clathrin包被小凹的边缘来完成这一功能。EPS15基因产生两种亚型，被认为分别定位于不同的亚细胞结构，并且可能与内体转运的不同方面有关（5）。人EPS15被定位于染色体1p31-p32，此区域常显示出若干非随机的染色体异常，包括神经母细胞瘤中的缺失、急性淋巴细胞和髓系白血病中的转位。两个t(1;11)(p32;q11)转位在罕见的髓系白血病中被描述，它会造成Eps15基因与HRX基因融合（6）。

1. Fazioli, F. et al. (1993) Mol Cell Biol 13, 5814-28.
2. Tebar, F. et al. (1996) J Biol Chem 271, 28727-30.
3. Parachoniak, C.A. and Park, M. (2009) J Biol Chem 284, 8382-94.
4. Torrisi, M.R. et al. (1999) Mol Biol Cell 10, 417-34.
5. Roxrud, I. et al. (2008) J Cell Biol 180, 1205-18.
6. Bernard, O.A. et al. (1994) Oncogene 9, 1039-45.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Chicken, Xenopus, Dog, Horse

Specificity / Sensitivity

Eps15 (D3K8R) Rabbit mAb recognizes endogenous levels of total Eps15 protein. Based upon sequence alignment, this antibody is predicted to react with both Eps15a and Eps15b. This antibody does not cross-react with Eps15R.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro630 of human Eps15 protein.

Background

Eps15 (EGFR pathway substrate 15) was originally discovered as a substrate for the kinase activity of EGFR (1). Eps15 has a tripartite structure comprising an amino terminal portion, which contains three evolutionarily conserved EH protein-protein interaction domains, a central putative coiled-coil region required for constitutive oligmerization, and a carboxy terminal domain containing multiple copies of the amino acid triplet Asp-Pro-Phe that constitute the AP2 binding domain. The carboxy terminal domain also contains two ubiquitin interaction motifs (UIMs), the last of which is indespensible for Eps15 binding to ubiquitin (1). Several lines of evidence support a role for Eps15 in clathrin-mediated endocytosis, including the endocytosis of synaptic vesicles. Eps15 binds to AP2 as well as other proteins involved in endocytosis and/or synaptic vesicle recycling, such as synaptojanin1 and epsin. Furthermore, Eps15 colocalizes with markers of the plasma membrane clathrin-coated pits and vesicles (2). Eps15 regulates the endosomal trafficking of c-Met (3) and EGFR (4), possibly by recruiting the ubiquitinated receptors to the rims of clathrin-coated pits through interaction between the ubiquitin tag and its UIMs. 
 
 The EPS15 gene yields two isoforms that are believed to reside in distinct subcellular locations and are thus implicated in different facets of endosomal trafficking (5). Human EPS15 has been mapped to chromosome 1p31-p32, a region displaying several nonrandom chromosomal abnormalities, including deletions in neuroblastoma and translocations in acute lymphoblastic and myeloid leukemias. Research has shown two translocations t(1;11)(p32;q11) are found in rare cases of myeloid leukemia where the Eps15 gene was fused to the HRX gene, resulting in two reciprocal fusion genes (6).

1. Fazioli, F. et al. (1993) Mol Cell Biol 13, 5814-28.
2. Tebar, F. et al. (1996) J Biol Chem 271, 28727-30.
3. Parachoniak, C.A. and Park, M. (2009) J Biol Chem 284, 8382-94.
4. Torrisi, M.R. et al. (1999) Mol Biol Cell 10, 417-34.
5. Roxrud, I. et al. (2008) J Cell Biol 180, 1205-18.
6. Bernard, O.A. et al. (1994) Oncogene 9, 1039-45.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.