MTMR3 Antibody

• 产品编号：CST-12443S      品牌：CST       原厂货号：12443S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MTMR3 Antibody能识别内源性水平的总MTMR3蛋白。此抗体与48kDa处的来源不明的蛋白有交叉反应。该多克隆抗体通过用合成肽免疫动物制备，该合成肽的序列与人MTMR3蛋白的Pro600周围的残基相一致。抗体通过蛋白A以及多肽亲和色谱法纯化。肌管素相关蛋白是在细胞信号和膜运输中新出现的一个磷酸酶类家族（1,2）。MTMR3（肌管素相关蛋白3），也称为FYVE-DSP1，包含一个氨基末端PH结构域和羧基末端FYVE结构域。MTMR3作为双特异性磷酸酶，是首次报道，它对磷酸化的丝氨酸、苏氨酸和酪氨酸残基有磷酸酶活性（3）。后续研究表明，MTMR3对磷酸肌醇有磷酸酶活性，包括磷酸肌醇-3-磷酸(PI3P)和3,5-二磷酸磷脂酰肌醇(PI(3,5)P2) (4)。由III级磷酸肌醇3-激酶Vps34引起的PI3P的积累，在自噬体形成过程中起到了关键作用（5）。由MTMR3引起的PI3P抑制，在抑制自噬体的形成中有重要作用（6）。

1. Robinson, F.L. and Dixon, J.E. (2006) Trends Cell Biol 16, 403-12.
2. Laporte, J. et al. (2002) Biochem Biophys Res Commun 291, 305-12.
3. Zhao, R. et al. (2000) Biochem Biophys Res Commun 270, 222-9.
4. Walker, D.M. et al. (2001) Curr Biol 11, 1600-5.
5. Simonsen, A. and Tooze, S.A. (2009) J Cell Biol 186, 773-82.
6. Taguchi-Atarashi, N. et al. (2010) Traffic 11, 468-78.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

MTMR3 Antibody recognizes endogenous levels of total MTMR3 protein. This antibody also cross-reacts with a protein of unknown origin at 48 kDa.

Background

Myotubularin-related proteins are a family of phosphatases with emerging roles in cellular signaling and membrane trafficking (1,2). MTMR3 (Myotubularin-related protein 3), also known as FYVE-DSP1, contains an amino terminal pleckstrin homology (PH) domain and a carboxyl terminal FYVE domain. MTMR3 was first reported as a dual-specific phosphatase, having phosphatase activity toward phosphorylated serine, threonine, and tyrosine residues (3). Subsequent research studies reported that MTMR3 has phosphatase activity toward phosphoinositides, including phosphatadylinositol-3-phosphate (PI3P) and phosphatidylinositol 3,5-bisphosphate (PI(3,5)P2) (4). Accumulation of PI3P by the class III phosphoinositide 3-kinase Vps34 is a key element in autophagosome formation (5). Inhibition of PI3P by MTMR3 can play an important role in suppressing autophagsome formation (6).

1. Robinson, F.L. and Dixon, J.E. (2006) Trends Cell Biol 16, 403-12.
2. Laporte, J. et al. (2002) Biochem Biophys Res Commun 291, 305-12.
3. Zhao, R. et al. (2000) Biochem Biophys Res Commun 270, 222-9.
4. Walker, D.M. et al. (2001) Curr Biol 11, 1600-5.
5. Simonsen, A. and Tooze, S.A. (2009) J Cell Biol 186, 773-82.
6. Taguchi-Atarashi, N. et al. (2010) Traffic 11, 468-78.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.