IL-1β (D4T2D) Rabbit mAb (Mouse Specific)

• 产品编号：CST-12426S      品牌：CST       原厂货号：12426S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IL-1β (D4T2D) Rabbit mAb (小鼠专用)兔单抗能够检测内源性鼠源IL-1β总蛋白水平。这种抗体可以检测500pg的成熟重组鼠源IL-1β。此单克隆抗体童工合成肽免疫动物制备，这种合成肽是鼠源IL-1β蛋白Val175周围的肽段。IL-1β是主要由激活的单核细胞和上皮细胞产生的促炎因子(1)。IL-1β的前体被caspase-1剪切，成熟的IL-1β紧接着被分泌(1-3)，目标细胞包括巨噬细胞和许多其它的细胞类型。IL-1β的信号传递过程包括IL-1β结合到IL-1附属蛋白上 (IL-1-AcP); 这种复合体紧接着结合到IL-1RI (1,2)。这种信号传递会激活MAP激酶和 NF-κB信号通路(1,2)。IL-1β也结合到IL-1RII，它缺乏细胞内的信号区域并因此作为高亲和性的诱捕受体。IL-1β结合到IL-1RI的过程能够被负调控因子IL-1R拮抗剂IL-1Ra所抑制。IL-1Ra结合到IL-1RI后无信号转导并且作为抑制剂阻止IL-1β信号通路。IL-1β在急性响应期和脓毒病中发挥了重要的作用(1-3)。

1. Dinarello, C.A. (1996) Blood 87, 2095-147.
2. Allan, S.M. et al. (2005) Nat Rev Immunol 5, 629-40.
3. Kramer, F. et al. (2008) Mol Immunol 45, 2678-89.

原厂资料：

Specificity / Sensitivity

IL-1β (D4T2D) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total mouse IL-1β protein. This antibody can detect 500 pg of mature recombinant mouse IL-1β.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val175 of mouse IL-1β protein.

Background

Interleukin-1β (IL-1β) is a proinflammatory cytokine produced predominantly by activated monocytes and epithelial cells (1). Precursor IL-1β is cleaved by caspase-1 and mature IL-1β is then secreted (1-3). Target cells include macrophages and many other cell types. Signaling by IL-1β involves IL-1β binding to IL-1 accessory protein (IL-1-AcP); the complex then binds to IL-1RI (1,2). Signaling occurs through activation of MAP kinase and NF-κB pathways (1,2). IL-1β also binds to IL-1RII, which lacks an intracellular signaling domain and thereby serves as a high affinity decoy receptor. IL-1β binding to IL-1RI is inhibited by the negative regulator, IL-1R antagonist (IL-1Ra). IL-1Ra binding to IL-1RI does not signal and serves to block IL-1β signaling. IL-1β plays critical roles in the acute phase response and sepsis (1-3).

1. Dinarello, C.A. (1996) Blood 87, 2095-147.
2. Allan, S.M. et al. (2005) Nat Rev Immunol 5, 629-40.
3. Kramer, F. et al. (2008) Mol Immunol 45, 2678-89.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody