HNF1α Antibody

• 产品编号：CST-12425S      品牌：Cell Signaling Technology       原厂货号：12425S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

HNF1α Antibody 能够检测内源性HNF1α总蛋白水平。该多克隆抗体是由合成的人源HNF1α蛋白N末端肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。肝细胞核因子1α（HNF1α也称为TCF1或MODY3）是一种转录因子，在肝脏基因表达的组织特异性调控中起重要作用（1）。有研究表明，HNF1α的异构突变与年青的成年发病型糖尿病（MODY）相关（2）。最近的研究表明，增加游离脂肪酸的浓度可以减少胰腺β-细胞中FoxA2/HNF3β和HNF1α的表达，造成它们的核排斥，导致一些代谢综合症的症状（3）。

1. Yamagata, K. et al. (1996) Nature 384, 455-8.
2. Lehto, M. et al. (1997) J Clin Invest 99, 582-91.
3. Ohtsubo, K. et al. (2011) Nat Med 17, 1067-75.

原厂资料：

Specificity / Sensitivity

HNF1α Antibody recognizes endogenous levels of total HNF1α protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HNF1α protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Hepatocyte nuclear factor 1α (HNF1α, also known as TCF1 or MODY3) is a transcription factor that plays a role in the tissue-specific regulation of liver gene expression (1). Research has shown that heterogeneous mutations of HNF1α are linked to maturity onset diabetes of the young (MODY) (2). Recent studies indicate that increased concentrations of free fatty acids can reduce the expression of FoxA2/HNF3β and HNF1α in pancreatic β-cells and lead to their nuclear exclusion, resulting in symptoms of several metabolic syndromes (3).

1. Yamagata, K. et al. (1996) Nature 384, 455-8.
2. Lehto, M. et al. (1997) J Clin Invest 99, 582-91.
3. Ohtsubo, K. et al. (2011) Nat Med 17, 1067-75.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.