Ezh2 (D2C9) XP??Rabbit mAb?(Biotinylated)

• 产品编号：CST-12408S      品牌：CST       原厂货号：12408S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

Ezh2 (D2C9) XP® Rabbit mAb 兔单抗(Biotinylated)能够检测内源性Ezh2总蛋白水平。该单克隆抗体是采用合成的与人源Ezh2蛋白Arg354残基周围序列相对应的肽段免疫动物而生产的。该CST抗体在最佳条件下偶联了生物素。此生物素标记抗体与未偶联的Ezh2 (D2C9) XP® Rabbit mAb #5246兔单抗具有相同的种属交叉反应性。olycomb group (PcG)蛋白是涉及维持数个发育调节基因的沉默水平，并且有助于维持细胞身份（cell identity）、细胞周期调节和肿瘤发生(1,2)。zeste homolog 2 (Ezh2)的增强子是一个该蛋白家族成员，它包含四种保守区域包括domain I、domain II、一个半胱氨酸富裕的氨基酸延伸序列和羧基端SET domain (3)。SET domain已经证明与histone methyltransferase (HMTase)活性有关联。此外，哺乳类Ezh2是一个组蛋白去乙酰酶复合物的成员之一，它在基因沉默中有功能，并且在染色质结构水平起着作用(4)。在体内，Ezh2复合物使histone H3在Lys9和27位点甲基化，这被认为是涉及到调节转录因子到特异的靶位点(5)。Ezh2蛋白在不同肿瘤形式中被下调，并且具有主要效应因子和肿瘤发生的调节因子的作用已经变成一越来越感兴趣的课题(6)。

1. Sellers, W.R. and Loda, M. (2002) Cancer Cell 2, 349-50.
2. Visser, H.P. et al. (2001) Br J Haematol 112, 950-8.
3. Chen, H. et al. (1996) Genomics 38, 30-7.
4. Tonini, T. et al. (2004) Oncogene 23, 4930-7.
5. Müller, J. et al. (2002) Cell 111, 197-208.
6. Kleer, C.G. et al. (2003) Proc Natl Acad Sci U S A 100, 11606-11.

原厂资料：

Specificity / Sensitivity

Ezh2 (D2C9) XP® Rabbit mAb (Biotinylated) recognizes endogenous levels of total Ezh2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg354 of human Ezh2 protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Ezh2 (D2C9) XP® Rabbit mAb #5246.

Background

The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6).

1. Sellers, W.R. and Loda, M. (2002) Cancer Cell 2, 349-50.
2. Visser, H.P. et al. (2001) Br J Haematol 112, 950-8.
3. Chen, H. et al. (1996) Genomics 38, 30-7.
4. Tonini, T. et al. (2004) Oncogene 23, 4930-7.
5. Müller, J. et al. (2002) Cell 111, 197-208.
6. Kleer, C.G. et al. (2003) Proc Natl Acad Sci U S A 100, 11606-11.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibodies.