PI3 Kinase Class II α (D3Q5B) Rabbit mAb

• 产品编号：CST-12402S      品牌：CST       原厂货号：12402S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PI3 Kinase Class II α (D3Q5B)Rabbit mAb可以识别内源性总的PI3K class II α蛋白。单克隆抗体由合成肽段免疫动物产生，该肽段与人PI3K class II α蛋白的Gly717邻近残基序列一致。Class II 磷脂酰肌醇3激酶 (PI3K)含有一个C末端C2结构域，使它区别于PI3K家族的其他class II亚型。C2结构域介导了蛋白和磷脂的结合（1,2）。PI3K Class II α 从磷脂酰肌醇（PI）和4-磷酸磷脂酰肌醇（PIP）生成3-磷酸磷脂酰肌醇 (PI(3)P) 和 3,4-二磷酸磷脂酰肌醇 (PI(3, 4)P2)。 PI3K Class II α位于多种细胞内结构，例如转运高尔基体网络，细胞内吞结构，网格蛋白有被小泡和核小（斑）点(1,4,5).研究表明PI3K Class II α 调控网格蛋白的聚集和分布，调控转运高尔基体网络内网格蛋白依赖的示踪和分类(5,6). PI3K Class II α也介导了葡萄糖转运蛋白GLUT4在胰岛素的刺激下转位到细胞膜的过程（7）。PI3K Class II α也被发现可以调控神经分泌颗粒的内吞组员（8）和血管平滑肌收缩（9）。与其他PI3K家族成员不同，PI3K Class II α对于PI3K的抑制剂wortmannin 和 LY294002没有那么敏感（3）。

1. Didichenko, S.A. and Thelen, M. (2001) J Biol Chem 276, 48135-42.
2. Stahelin, R.V. et al. (2006) J Biol Chem 281, 39396-406.
3. Domin, J. et al. (1997) Biochem J 326 ( Pt 1), 139-47.
4. Domin, J. et al. (2000) J Biol Chem 275, 11943-50.
5. Gaidarov, I. et al. (2001) Mol Cell 7, 443-9.
6. Gaidarov, I. et al. (2005) J Biol Chem 280, 40766-72.
7. Falasca, M. et al. (2007) J Biol Chem 282, 28226-36.
8. Wen, P.J. et al. (2008) Mol Biol Cell 19, 5593-603.
9. Yoshioka, K. et al. (2007) Mol Pharmacol 71, 912-20.

原厂资料：

Specificity / Sensitivity

PI3 Kinase Class II α (D3Q5B) Rabbit mAb recognizes endogenous levels of total PI3K class II α protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly717 of human PI3K class II α protein.

Background

Class II phosphatidylinositol 3-kinases (PI3K) contain a C-terminal C2 domain that is unique to the class II isoforms of the PI3K family. This C2 domain mediates protein and phospholipid binding acitivities (1,2). PI3K Class II α generates phosphatidylinositol 3-phosphate (PIP3) and phosphatidylinositol 3,4-bisphosphate (PI(3, 4)P2) from phosphatidylinositol and phosphatidylinositol 4-phosphate (3). PI3K Class II α is located in various intracellular locations such as the trans-Golgi network, endocytic compartments, clathrin-coated vesicles, and nuclear speckles (1,4,5). Research studies have indicated that PI3K Class II α regulates the assembly and distribution of clathrin, resulting in the modulation of clathrin-dependent trafficking and sorting within the trans Golgi network (5,6). PI3K Class II α also mediates translocation of the glucose transporter GLUT4 to the plasma membrane in response to insulin (7). PI3K Class II α has also been shown to regulate neurosecretory granule exocytosis (8) and vascular smooth muscle contraction (9). Unlike other PI3K family members, PI3K Class II α is less sensitive to the PI3K inhibitors wortmannin and LY294002 (3).

1. Didichenko, S.A. and Thelen, M. (2001) J Biol Chem 276, 48135-42.
2. Stahelin, R.V. et al. (2006) J Biol Chem 281, 39396-406.
3. Domin, J. et al. (1997) Biochem J 326 ( Pt 1), 139-47.
4. Domin, J. et al. (2000) J Biol Chem 275, 11943-50.
5. Gaidarov, I. et al. (2001) Mol Cell 7, 443-9.
6. Gaidarov, I. et al. (2005) J Biol Chem 280, 40766-72.
7. Falasca, M. et al. (2007) J Biol Chem 282, 28226-36.
8. Wen, P.J. et al. (2008) Mol Biol Cell 19, 5593-603.
9. Yoshioka, K. et al. (2007) Mol Pharmacol 71, 912-20.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.