ROS1 (D4D6?) Rabbit mAb (Biotinylated)

• 产品编号：CST-12368S      品牌：CST       原厂货号：12368S
• 产品分类：抗体 > 一抗 > 表位标签和报告基因特异性抗体
• 应用分类：

描述：

ROS1 (D4D6) 兔单抗(生物素标记)能够识别内源性水平的总ROS1蛋白。当用western blot进行分析时，该抗体不与其他相关蛋白发生交叉反应。该单克隆抗体是通过用一个蛋白免疫动物而制备的，该蛋白是人ROS1蛋白羧基末端区域的残基。该Cell Signaling Technology公司的抗体在最佳条件下与生物素结合。该抗体与非结合的ROS1 (D4D6)兔单抗 #3287预期表现出同一物种的交叉反应。ROS1是胰岛素受体家族的一个孤儿受体酪氨酸激酶，被初步确定为来自UR2肉瘤病毒的v-ros的同系物（1）。ROS1由一个大的胞外结构域，一个跨膜结构域和一个细胞内激酶结构域组成，其中胞外结构域由6个纤连蛋白重复序列组成。虽然ROS1的功能尚未定义，但已证明它在附睾上皮细胞分化中发挥重要的作用（2）。通过在胶质母细胞瘤中研究，初步确定第一个ROS1的致癌融合, FIG-ROS1（3），在随后的研究中发现这种融合在胆管癌（4），卵巢癌（5）和非小细胞肺癌（NSCLC）（6）中都存在。在非小细胞肺癌（1.6％的发生频率）中，研究者已经发现额外的致癌ROS1融合蛋白，其中ROS1的激酶结构域与若干种不同蛋白的氨基末端区域融合，这些蛋白包括CD74和SLC34A2（6-8）。ROS1融合蛋白激活SHP-2磷酸酶，PI3K/Akt/mTOR，ERK和STAT3通路（3,4,9）。

1. Matsushime, H. et al. (1986) Mol Cell Biol 6, 3000-4.
2. Yeung, C.H. et al. (1999) Biol Reprod 61, 1062-9.
3. Charest, A. et al. (2003) Genes Chromosomes Cancer 37, 58-71.
4. Gu, T.L. et al. (2011) PLoS One 6, e15640.
5. Birch, A.H. et al. (2011) PLoS One 6, e28250.
6. Rimkunas, V.M. et al. (2012) Clin Cancer Res 18, 4449-57.
7. Rikova, K. et al. (2007) Cell 131, 1190-203.
8. Stumpfova, M. and Jänne, P.A. (2012) Clin Cancer Res 18, 4222-4.
9. Jun, H.J. et al. (2012) Cancer Res 72, 3764-74.

原厂资料：

Specificity / Sensitivity

ROS1 (D4D6) Rabbit mAb (Biotinylated) recognizes endogenous levels of total ROS1 protein. This antibody does not cross-react with other related proteins when analyzed by western blot.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a protein corresponding to residues in the carboxy terminal domain of the human ROS1 protein.

Description

This Cell Signaling Technology antibody is conjugated to biotin under optimal conditions. The biotinylated antibody is expected to exhibit the same species cross-reactivity as the unconjugated ROS1 (D4D6) Rabbit mAb #3287.

Background

ROS1, an orphan receptor tyrosine kinase of the insulin receptor family, was initially identified as a homolog of v-ros from the UR2 sarcoma virus (1). ROS1 consists of a large extracellular domain that is composed of six fibronectin repeats, a transmembrane domain, and an intracellular kinase domain. While the function of ROS1 is undefined, it has been shown to play an important role in differentiation of epididymal epithelium (2). The first oncogenic fusion of ROS1, FIG-ROS1, was initially identified by research studies in glioblastoma (3), and subsequent studies have found this fusion in cholangiocarcinoma (4), ovarian cancer (5) and non-small cell lung cancer (NSCLC) (6). Investigators have found additional oncogenic ROS1 fusion proteins in NSCLC (at a frequency of ~1.6%), where the ROS1 kinase domain is fused to the amino-terminal region of a number of different proteins, including CD74 and SLC34A2 (6-8). ROS1 fusion proteins activate the SHP-2 phosphatase, PI3K/Akt/mTOR, Erk, and Stat3 pathways (3,4,9).

1. Matsushime, H. et al. (1986) Mol Cell Biol 6, 3000-4.
2. Yeung, C.H. et al. (1999) Biol Reprod 61, 1062-9.
3. Charest, A. et al. (2003) Genes Chromosomes Cancer 37, 58-71.
4. Gu, T.L. et al. (2011) PLoS One 6, e15640.
5. Birch, A.H. et al. (2011) PLoS One 6, e28250.
6. Rimkunas, V.M. et al. (2012) Clin Cancer Res 18, 4449-57.
7. Rikova, K. et al. (2007) Cell 131, 1190-203.
8. Stumpfova, M. and Jänne, P.A. (2012) Clin Cancer Res 18, 4222-4.
9. Jun, H.J. et al. (2012) Cancer Res 72, 3764-74.

注意事项：

Storage: Supplied in 136 mM NaCl, 2.6 mM KCI, 12 mM
sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50%
glycerol. Store at –20°C. Do not aliquot the antibodies.