VCAM-1 Antibody

• 产品编号：CST-12367S      品牌：Cell Signaling Technology       原厂货号：12367S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

VCAM-1抗体能识别内源性VCAM-1总蛋白水平，此抗体与来源不明的48和49kDa的蛋白有交叉反应。此多克隆抗体通过用合成肽免疫动物制备，该合成肽是人VCAM-1蛋白Pro354周围的残基。抗体通过蛋白A以及多肽亲和色谱法纯化。VCAM-1（血管细胞粘附分子-1）是一种跨膜糖蛋白，包含多个氨基端的细胞外的Ig样结构域（跨膜区）和一个短的羧基端的胞浆区（1）。选择性剪接产生两个VCAM-1亚型（2）。VCAM-1在感染和炎性疾病发生过程中有特征性作用。VCAM-1在内皮细胞中的表达由炎性细胞因子诱导，包括TNF-α 和IL-1β (1)。内皮细胞中的VCAM-1和白细胞中的整联蛋白VLA-4 (α4β1)相互作用，介导循环白细胞从血液穿过内皮进入组织的迁移（3）。

1. Osborn, L. et al. (1989) Cell 59, 1203-11.
2. Hession, C. et al. (1991) J Biol Chem 266, 6682-5.
3. Elices, M.J. et al. (1990) Cell 60, 577-84.

原厂资料：

Specificity / Sensitivity

VCAM-1 Antibody recognizes endogenous levels of total VCAM-1 protein. This antibody cross-reacts with proteins of unknown origin 48 and 49 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro354 of human VCAM-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

VCAM-1 (vascular cell adhesion molecule-1) is a transmembrane glycoprotein containing multiple amino-terminal extracellular Ig-like domains, a transmembrane domain, and a short carboxy-terminal cytoplasmic domain (1). Alternative splicing generates two isoforms of VCAM-1 (2). The role of VCAM-1 during infection and inflammatory diseases is well characterized. Expression of VCAM-1 is induced in endothelial cells by inflammatory cytokines including TNF-α and IL-1β (1). VCAM-1 on endothelial cells interacts with the integrin VLA-4 (α4β1) on leukocytes to mediate migration of circulating leukocytes from the blood across the endothelium and into tissues (3).

1. Osborn, L. et al. (1989) Cell 59, 1203-11.
2. Hession, C. et al. (1991) J Biol Chem 266, 6682-5.
3. Elices, M.J. et al. (1990) Cell 60, 577-84.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.