TPH-1 (D10C10) Rabbit mAb

• 产品编号：CST-12339S      品牌：CST       原厂货号：12339S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

TPH-1 (D10C10) Rabbit mAb兔单抗能够检测内源性的TPH-1总蛋白。该单克隆抗体是由合成的人源TPH-1蛋白C末端肽段免疫动物而制备的。色氨酸羟化酶（TPH）是五羟色胺生物合成中的限速酶（1），该过程是通过转换色氨酸到5-羟基-L-色氨酸实现（2）。TPH存在两个亚型：TPH-1主要在周围表达，而TPH-2的表达被限制在神经细胞和中枢神经系统（3）。TPH-1是在胃肠道的肠嗜铬细胞合成了存在于人体内的5 - 羟色胺。tph1基因的缺失会导致组织和循环系统中5-羟色胺的含量较低（4）。

1. Hale, M.W. et al. (2011) J Chem Neuroanat 41, 219-26.
2. Silva, S.R. et al. (2011) Anticancer Res 31, 1153-60.
3. Walther, D.J. et al. (2003) Science 299, 76.
4. Amireault, P. et al. (2011) Proc Natl Acad Sci U S A 108, 13141-6.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human

Specificity / Sensitivity

TPH-1 (D10C10) Rabbit mAb recognizes endogenous levels of total TPH-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human TPH-1 protein.

Background

Tryptophan hydroxylase (TPH) is the rate-limiting enzyme in the biosynthesis of serotonin (1) by converting tryptophan to 5-hydroxy-L-tryptophan (2). Two isoforms of TPH exist: TPH-1 is mainly expressed in the periphery, whereas the expression of TPH-2 is restricted to neuronal cells and the central nervous system (3). Most of the serotonin found throughout the body is synthesized by TPH-1 in enterochromaffin cells of the gastrointestinal tract. Targeted disruption of the tph1 gene results in low levels of circulating and tissue serotonin (4).

1. Hale, M.W. et al. (2011) J Chem Neuroanat 41, 219-26.
2. Silva, S.R. et al. (2011) Anticancer Res 31, 1153-60.
3. Walther, D.J. et al. (2003) Science 299, 76.
4. Amireault, P. et al. (2011) Proc Natl Acad Sci U S A 108, 13141-6.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.