SignalSilence? Miz-1 siRNA I

• 产品编号：CST-12335S      品牌：CST       原厂货号：12335S
• 产品分类：DNA和RNA纯化 > RNA干扰 > RNAi Oligos > siRNA
• 应用分类：

描述：

SignalSilence^® Miz-1 siRNA I能够抑制人、小鼠和猴的Miz-1的表达。来自Cell Signaling Technology (CST)的SignalSilence^® Miz-1 siRNA I可以帮助研究者通过RNA干扰特异性地抑制Miz-1的表达，这种方法可以通过将双链RNA分子传递到细胞内从而使基因表达有选择的沉默。来自CST的所有的SignalSilence^® siRNA产品都是经过内部严格检测的，并且通过Western blot 分析证明确实能够减少目的蛋白的表达。通过三苯甲基分析每个碱基以监测寡核苷酸的合成，确保合适的配对效率。随后寡核苷酸通过亲和固相萃取法纯化。退火的RNA双链通过质谱分析来证实其精确的组成。每一批产品都通过质谱分析与前面的产品进行比较，来保证不同批次之间的最大一致性。CST推荐使用100 nM SignalSilence^® Miz-1 siRNA I进行转染，48到72小时后对细胞进行裂解。转染步骤按照转染试剂说明书提供的步骤进行。遇到任何使用方面的问题，请随时联系CST。每小瓶可供100次转染，每次转染量相当于在转染24孔板时，每孔总体积为300μl培养基中siRNA的终浓度为100nM。Miz-1 (Zbtb17)是一种痘病毒和锌指(POZ)转录因子，有一个氨基端的BTB/POZ结构域和13个羧基端的锌指结构域。Miz-1通过CDK抑制蛋白(p15，INK4B和p21 Waf1/Cip1)的活化在细胞周期控制中有重要作用（1-4）。与Myc的直接相互作用能抑制Miz-1的转录活性（1-4）。在DNA损伤中，Myc通过Miz-1募集到p21 Waf1/Cip1启动子上，并阻止p53介导的p21 Waf1/Cip1的诱导表达，最终引起p53介导的细胞凋亡而不是细胞周期停滞（4）。Miz-1在淋巴细胞发育过程中也发挥作用。在B和T细胞发育中，Miz-1抑制细胞因子信号抑制蛋白1(SOCS1)的表达，使信号穿过IL-7受体，并使促存活蛋白Bcl-2表达上调（5,6）。

1. Peukert, K. et al. (1997) EMBO J 16, 5672-86.
2. Seoane, J. et al. (2001) Nat Cell Biol 3, 400-8.
3. Staller, P. et al. (2001) Nat Cell Biol 3, 392-9.
4. Seoane, J. et al. (2002) Nature 419, 729-34.
5. Kosan, C. et al. (2010) Immunity 33, 917-28.
6. Saba, I. et al. (2011) Blood 117, 3370-81.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

SignalSilence® Miz-1 siRNA I inhibits human, mouse, rat, and monkey Miz-1 expression.

Description

SignalSilence® Miz-1 siRNA I from Cell Signaling Technology (CST) allows the researcher to specifically inhibit Miz-1 expression using RNA interference, a method whereby gene expression can be selectively silenced through the delivery of double stranded RNA molecules into the cell. All SignalSilence® siRNA products from CST are rigorously tested in-house and have been shown to reduce target protein expression by western analysis.

Quality Control

Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.

Directions for Use

CST recommends transfection with 100 nM SignalSilence® Miz-1 siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use. 
 Each vial contains the equivalent of 100 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

Background

Miz-1 (Zbtb17) is a poxvirus and zinc finger (POZ) transcription factor with an amino-terminal BTB/POZ domain and 13 carboxy-terminal zinc finger domains. Miz-1 plays a key role in cell cycle control through activation of the cyclin-dependent kinase inhibitors p15, INK4B, and p21 Waf1/Cip1 (1-4). The transcriptional activity of Miz-1 is repressed through direct interaction with Myc (1-4). In the presence of DNA damage, Myc is recruited to the p21 Waf1/Cip1 promoter by Miz-1 and blocks p53-mediated induction of p21 Waf1/Cip1, ultimately resulting in p53-mediated apoptosis rather than cell cycle arrest (4). Miz-1 also plays a role during lymphocyte development. In developing B and T cells, Miz-1 represses suppressor of cytokine signaling 1 (SOCS1) expression, which enables signaling through the IL-7 receptor and upregulation of the pro-survival protein Bcl-2 (5,6).

1. Peukert, K. et al. (1997) EMBO J 16, 5672-86.
2. Seoane, J. et al. (2001) Nat Cell Biol 3, 400-8.
3. Staller, P. et al. (2001) Nat Cell Biol 3, 392-9.
4. Seoane, J. et al. (2002) Nature 419, 729-34.
5. Kosan, C. et al. (2010) Immunity 33, 917-28.
6. Saba, I. et al. (2011) Blood 117, 3370-81.

注意事项：

Storage: Miz-1 siRNA I is supplied in RNAse-free water. Aliquot and store at -20ºC.