Thap11/Ronin Antibody

• 产品编号：CST-12305S      品牌：CST       原厂货号：12305S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Thap11/Ronin抗体可以识别内源性的总Thap11/Ronin蛋白。多抗由合成肽段免疫动物产生，该肽段与人Thap11蛋白邻近羧基末端氨基酸残基序列一致。抗体由蛋白A和肽段亲和层析技术纯化得到。Thanatos-associated protein (Thap)蛋白是一种细胞因子在进化中保守，含有类似于果蝇P因子转座酶的DNA结合结构域（1）。已知有12种人类Thap蛋白，都作为位点特异性DNA结合因子，涉及转录调控，细胞增殖，染色体修饰和凋亡（2-4）。人Thap11被认为能够通过抑制c-Myc转录而抑制细胞生长（5）。Thap11的小鼠同源物，Ronin，被认为是小鼠胚胎干细胞发育过程中的关键蛋白（6）。

1. Roussigne, M. et al. (2003) Trends Biochem Sci 28, 66-9.
2. Cayrol, C. et al. (2007) Blood 109, 584-94.
3. Macfarlan, T. et al. (2005) J Biol Chem 280, 7346-58.
4. Roussigne, M. et al. (2003) Oncogene 22, 2432-42.
5. Zhu, C.Y. et al. (2009) Cell Death Differ 16, 395-405.
6. Volchegorskiĭ, I.A. et al. Vopr Med Khim 37, 86-9.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey

Specificity / Sensitivity

Thap11/Ronin Antibody recognizes endogenous levels of total Thap11/Ronin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Thap11 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Thanatos-associated protein (Thap) proteins are a family of cellular factors that are characterized by an evolutionarily conserved protein motif similar to the DNA-binding domain of Drosophila P element transposase (1). There are 12 known human Thap proteins that all act as site-specific DNA-binding factors involved in transcriptional regulation, cell proliferation, chromatin modification, and apoptosis (2-4). Human Thap11 has been shown to suppress cell growth through transcriptional suppression of c-Myc (5). The mouse homolog of Thap11, Ronin, has been identified as an essential factor underlying embryogenesis in mouse embryonic stem cells (6).

1. Roussigne, M. et al. (2003) Trends Biochem Sci 28, 66-9.
2. Cayrol, C. et al. (2007) Blood 109, 584-94.
3. Macfarlan, T. et al. (2005) J Biol Chem 280, 7346-58.
4. Roussigne, M. et al. (2003) Oncogene 22, 2432-42.
5. Zhu, C.Y. et al. (2009) Cell Death Differ 16, 395-405.
6. Volchegorskiĭ, I.A. et al. Vopr Med Khim 37, 86-9.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.