PhosphoPlus? Chk2 (Thr68) Antibody Duet

• 产品编号：CST-12298S      品牌：CST       原厂货号：12298S
• 产品分类：抗体 > 一抗 > 抗体对
• 应用分类：

其它组分：

描述：

Cell Signaling Technology (CST)生产的PhosphoPlus® Duets提供了一种方法以衡量蛋白的活性状态。每一个Duet都含有一个激活状态和对应感兴趣的靶蛋白的总蛋白抗体。这些选自CST产品的抗体能够在特定的应用中提供良好的表现。Chk2是出芽酵母Rad53和裂殖酵母Cds1检测点激酶的哺乳动物同源物（1-3）。Chk2的氨基端有一组七个丝氨酸或苏氨酸残基（Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, 和 Thr68）的结构，每个结构后都有一个谷氨酸（SQ或TQ基序）。这被认为是ATM/ATR激酶的最佳磷酸化位点（4,5）。在离子辐射（IR），UV辐射或羟基脲处理引起DNA损伤后，该区域的68位苏氨酸和其他位点可以被ATM/ATR磷酸化（5-7）。因此SQ/TQ结构域似乎具有调控功能。68位苏氨酸磷酸化是后续激活步骤的前提，会导致Chk2激酶结构域的383和387位苏氨酸自磷酸化（8）。

1. Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415.
2. Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665.
3. Murakami, H. and Okayama, H. (1995) Nature 374, 817-819.
4. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.
5. Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394.
6. Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.
7. Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936.
8. Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.

原厂资料：

Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).

1. Allen, J.B. et al. (1994) Genes Dev. 8, 2401-2415.
2. Weinert, T.A. et al. (1994) Genes Dev. 8, 652-665.
3. Murakami, H. and Okayama, H. (1995) Nature 374, 817-819.
4. Kastan, M.B. and Lim, D.S. (2000) Nat. Rev. Mol. Cell Biol. 1, 179-186.
5. Matsuoka, S. et al. (2000) Proc. Natl. Acad. Sci. USA 97, 10389-10394.
6. Melchionna, R. et al. (2000) Nat. Cell Biol. 2, 762-765.
7. Ahn, J.Y. et al. (2000) Cancer Res. 60, 5934-5936.
8. Lee, C.H. and Chung, J.H. (2001) J. Biol. Chem. 276, 30537-30541.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.