Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb

• 产品编号：CST-12280S      品牌：CST       原厂货号：12280S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9)Rabbit mAb兔单抗能够识别内源性Ser189 (MKK3)或Ser207 (MKK6)位点被磷酸化的MKK3和MKK6蛋白。单克隆抗体是采用合成的与人源MKK3蛋白Ser189位点和人MKK6蛋白的Ser207位点附近氨基酸一致的序列免疫动物而产生的。MKK3和MKK6是两种密切相关的双特异性蛋白激酶，能够激活p38 MAPK（1-5）。MKK3和MKK6能够通过磷酸化p38 MAPK的活化位点Thr-Gly-Tyr从而使p38 MAPK激活，但不能磷酸化或激活Erk1/2或SAPK/JNK。p38 MAPK的磷酸化能够显著提高其磷酸化蛋白底物的能力，这些底物包括ATF-2和Elk-1。MKK3和MKK6能被不同形式的细胞压力和炎症细胞因子激活（4,5）。通过磷酸化MKK3的Ser189和Thr222位点(2)以及MKK6的Ser207和Thr211位点（4,5）可以激活MKK3和MKK6。

1. Derijard, B. et al. (1995) Science 267, 682-685.
2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Rat, Monkey, Zebrafish, Bovine, Guinea Pig

Specificity / Sensitivity

Phospho-MKK3 (Ser189)/MKK6 (Ser207) (D8E9) Rabbit mAb recognizes endogenous levels of MKK3 and MKK6 proteins only when phosphorylated at Ser189 (MKK3) or Ser207 (MKK6).

Source / Purification

Monoclonal antibody is produced by immunizing animals with synthetic peptides corresponding to residues surrounding Ser189 of human MKK3 protein and Ser207 of human MKK6 protein.

Background

MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase (1-5). MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines (4,5). Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 (2) and Ser207 and Thr211 on MKK6 (4,5).

1. Derijard, B. et al. (1995) Science 267, 682-685.
2. Raingeaud, J. et al. (1995) J. Biol. Chem. 270, 7420-7426.
3. Sluss, H.K. et al. (1994) Mol. Cell. Biol. 14, 8376-8384.
4. Raingeaud, J. et al. (1996) Mol. Cell. Biol. 16(3), 1247-1255.
5. Han, J. et al. (1996) J. Biol. Chem. 271, 2886-2891.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.