β1-Adrenergic Receptor Antibody

• 产品编号：CST-12271S      品牌：CST       原厂货号：12271S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

β1-Adrenergic Receptor Antibody兔多抗识别内源性水平总人β1-肾上腺素受体蛋白。该抗体不与β2-肾上腺素受体发生交叉反应。该多克隆抗体通过用合成肽免疫动物制备，该合成肽与人β1-肾上腺素受体蛋白甘氨酸（406位）附近的残基一样。抗体由蛋白A和肽亲和层析纯化。β1-肾上腺素受体(β1AR)）是一种G蛋白偶联受体（GPCR），参与心血管功能的调节（1）。与β2AR和β1AR一起，是心脏中主要的肾上腺素受体。β1AR由儿茶酚胺激活并与Gαs蛋白耦联，激活腺苷酸环化酶，增加细胞内cAMP的水平(2)。β-受体阻滞剂（βAR受体拮抗剂），是治疗心血管疾病的主要类别之一，主要通过预防儿茶酚胺与β1AR结合而起作用（3）。

1. Brodde, O.E. and Michel, M.C. (1999) Pharmacol Rev 51, 651-90.
2. Rockman, H.A. et al. (2002) Nature 415, 206-12.
3. Frishman, W.H. Am J Ther 15, 565-76.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

β1-Adrenergic Receptor Antibody recognizes endogenous levels of total human β1-Adrenergic Receptor protein. This antibody does not cross-react with β2-Adrenergic Receptor.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly406 of human β1-Adrenergic Receptor protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

β1-Adrenergic Receptor (β1AR) is a G protein-coupled receptor (GPCR) involved in the regulation of cardiovascular functions (1). Together with β2AR, β1AR is a major βAR in the heart. β1AR is activated by catecholamines and couples to Gαs protein, activating adenylate cyclase and increasing intracellular cAMP levels (2). Beta-blockers (βAR antagonists), one of the major class of therapeutics in cardiovascular medicine, act mostly by preventing catecholamine binding to β1AR (3).

1. Brodde, O.E. and Michel, M.C. (1999) Pharmacol Rev 51, 651-90.
2. Rockman, H.A. et al. (2002) Nature 415, 206-12.
3. Frishman, W.H. Am J Ther 15, 565-76.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.