Plectin-1 (D6A11) Rabbit mAb

• 产品编号：CST-12254S      品牌：CST       原厂货号：12254S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

lectin-1 (D6A11) Rabbit mAb 用于检测内源性的总Plectin-1蛋白。该单克隆抗体由人plectin-1蛋白Leu2980附近的残基合成多肽免疫动物产生。网格蛋白是一种大的、广泛表达的蛋白，在中间丝和肌动蛋白细胞骨架中交联，通过机械作用稳定细胞核组织。网格蛋白也在肌动蛋白动力学中起作用，并在信号转导分子中起支架作用(1)。网格蛋白在稳定半桥粒中起重要作用，交联半桥粒于中间丝网络中。研究表明网格蛋白和其他编码半桥粒基因的蛋白变化可以引起单纯型大疱性表皮松解症，该疾病皮肤脆弱并频繁起疱(1,2)。网格蛋白通过结合和隔离PACK1-激活C激酶1受体调节PKC信号(3,4)。网格蛋白也在细胞角蛋白结构和细胞压力应答中起调节作用(4)，通过细胞因子受体CXCR4的传递信号，并调节AMP-活化蛋白激酶（AMPK）活性和鼠肌管的信号传导(6)。

1. Wiche, G. (1998) J Cell Sci 111 ( Pt 17), 2477-86.
2. Pfendner, E. et al. (2005) Exp Dermatol 14, 241-9.
3. Osmanagic-Myers, S. and Wiche, G. (2004) J Biol Chem 279, 18701-10.
4. Osmanagic-Myers, S. et al. (2006) J Cell Biol 174, 557-68.
5. Ding, Y. et al. (2008) Exp Cell Res 314, 590-602.
6. Gregor, M. et al. (2006) J Cell Sci 119, 1864-75.

原厂资料：

Specificity / Sensitivity

Plectin-1 (D6A11) Rabbit mAb recognizes endogenous levels of total plectin-1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu2980 of human plectin-1 protein.

Background

Plectin is a large, widely expressed protein that crosslinks the intermediate filament and actin cytoskeleton, mechanically stabilizing cells and tissues. Plectin also plays a role in the regulation of actin dynamics and acts as a scaffold for signaling molecules (1). Plectin is important in the stabilization of hemidesmosomes, crosslinking them to the intermediate filament network. Research studies have shown that mutations in plectin and other genes coding for hemidesmosomal proteins can cause epidermolysis bullosa, a condition manifested by fragile skin and frequent blistering (1,2). Plectin modulates signals to PKC through binding and sequestration of RACK1, the receptor for activated C kinase 1 (3,4). Plectin is also involved in the regulation of cytokeratin architecture and cell stress response (4), signaling through the chemokine receptor CXCR4 (5) and regulation of AMP-activated protein kinase (AMPK) activity and signaling in mouse myotubes (6).

1. Wiche, G. (1998) J Cell Sci 111 ( Pt 17), 2477-86.
2. Pfendner, E. et al. (2005) Exp Dermatol 14, 241-9.
3. Osmanagic-Myers, S. and Wiche, G. (2004) J Biol Chem 279, 18701-10.
4. Osmanagic-Myers, S. et al. (2006) J Cell Biol 174, 557-68.
5. Ding, Y. et al. (2008) Exp Cell Res 314, 590-602.
6. Gregor, M. et al. (2006) J Cell Sci 119, 1864-75.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody