Calreticulin (D3E6) XP? Rabbit mAb

• 产品编号：CST-12238P      品牌：Cell Signaling Technology       原厂货号：12238P
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Calreticulin (D3E6) XP® Rabbit mAb兔单抗识别内源性的总钙网蛋白。该单克隆抗体通过用合成肽免疫动物制备，该合成肽与人钙网蛋白靠近氨基末端的残基一样。钙是一种通用的信号传导分子，参与许多细胞功能，如细胞运动、代谢、蛋白质修饰、蛋白质折叠和细胞凋亡。钙存储于内质网（ER），在内质网内与钙离子结合分子伴侣如钙联接蛋白和钙网蛋白缓冲，并通过IP3受体通道释放（1）。钙网蛋白也作为内质网的分子伴侣起作用，以确保新合成糖蛋白的正确折叠和质量控制。因此，推测钙网蛋白不会改变蛋白质的折叠，而是在恰当的时间点调节有效折叠和亚基组装。此外，在内质网中，钙网蛋白以非天然构象保存，并对其进行靶向降解（2,3）。

1. Groenendyk, J. et al. (2004) Mol Cells 17, 383-9.
2. Nauseef, W.M. et al. (1995) J Biol Chem 270, 4741-7.
3. Williams, D.B. (2006) J Cell Sci 119, 615-23.

原厂资料：

Specificity / Sensitivity

Calreticulin (D3E6) XP® Rabbit mAb recognizes endogenous levels of total calreticulin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human calreticulin protein.

Background

Calcium is a universal signaling molecule involved in many cellular functions such as cell motility, metabolism, protein modification, protein folding, and apoptosis. Calcium is stored in the endoplasmic reticulum (ER), where it is buffered by calcium binding chaperones such as calnexin and calreticulin, and is released via the IP3 Receptor channel (1). Calreticulin also functions as an ER chaperone that ensures proper folding and quality control of newly synthesized glycoproteins. As such, calreticulin presumably does not alter protein folding but regulates proper timing for efficient folding and subunit assembly. Furthermore, calreticulin retains proteins in non-native conformation within the ER and targets them for degradation (2,3).

1. Groenendyk, J. et al. (2004) Mol Cells 17, 383-9.
2. Nauseef, W.M. et al. (1995) J Biol Chem 270, 4741-7.
3. Williams, D.B. (2006) J Cell Sci 119, 615-23.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody