Mouse IL-3 Neutralizing (D6C1) Rabbit mAb

• 产品编号：CST-12222S      品牌：CST       原厂货号：12222S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

小鼠IL-3 Neutralizing (D6C1) Rabbit mAb（中和型兔单抗)能够结合鼠源IL-3并且中和其在BaF3细胞增殖过程中的活性。这种抗体不会与人源IL-3，人源IL-5，人源GM-CSF以及鼠源GM-CSF发生交叉反应。IL-3是通过T细胞、肌肉细胞和噬曙红细胞而产生的(1)，目标细胞包括造血原细胞、嗜中性粒细胞、巨噬细胞、肌肉细胞、噬曙红细胞、淋巴细胞和色调微红细胞(1)。IL-3受体是IL-3特异性的α链和常规的β链，βc结合的异源二聚体，βc同样也在GM-CSF和IL-5中被使用(1)。IL-3结合同源二聚体受体能够诱导Jak2以及PI3K/Akt通路的活化，以及Stat1/3/5/6的磷酸化(1)。IL-3可能在与哮喘气道炎症相关的疾病发展中发挥着至关重要的作用(3-5)。

原厂资料：

Specificity / Sensitivity

Mouse IL-3 Neutralizing (D6C1) Rabbit mAb binds to mouse IL-3 and neutralizes its effects in a BaF3 cell proliferation assay. This antibody does not cross-react with human IL-3, human IL-5, human GM-CSF, or mouse GM-CSF.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant mouse IL-3 protein.

Description

Neutralizing antibodies can be used to inhibit normal biological function through their binding to biological molecules. These reagents can be used to determine the effects that a particular molecule has in biological systems. Mouse IL-3 Neutralizing (D6C1) Rabbit mAb has been shown to neutralize the proliferation of IL-3-dependent BaF3 cells in vitro with an ND50 in the range of 0.5-3.5 µg/ml.

注意事项：

Formulation: Lyophilized from a 0.2 µm filtered solution in
10 mM HEPES with trehalose.
Reconstitution: Add sterile 10 mM HEPES pH 7.0 to a final
concentration of greater than 50 µg/ml. Solubilize for 20 min at
room temperature with occasional gentle vortexing.
Storage: Store Lyophilized material at -20ºC. After reconstitu-
tion, recommended storage at 4ºC for 1 month or -20ºC for 6
months. Avoid repeated freeze/thawing.