SHANK2 Antibody

• 产品编号：CST-12218S      品牌：CST       原厂货号：12218S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

SHANK2 Antibody兔多抗能够检测内源性的SHANK2总蛋白。该多克隆抗体是由合成的人源SHANK2蛋白缬氨酸(537位)肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。SHANK家族蛋白，也被称为富含脯氨酸的突触相关蛋白，包含SHANK1 SHANK2，SHANK3。SHANK蛋白作为神经元突触后致密物（PSD）的支架（1），在发育中兴奋性突触组装PSD时发挥了关键作用（2）。虽然招募SHANK蛋白到突触不依赖其与Homer的相互作用（3），SHANK1适当的突触靶向性是被其与PDZ结构域和PSD蛋白之间的相互作用所介导的（4）。在突触中，SHANK蛋白与NMDA受体和代谢型谷氨酸受体复合物相互作用（5）。研究指出，SHANK蛋白与孤独症和神经退行性疾病相关（6）。

1. Grabrucker, A.M. et al. (2011) Trends Cell Biol 21, 594-603.
2. Boeckers, T.M. et al. (1999) J Neurosci 19, 6506-18.
3. Boeckers, T.M. et al. (2005) J Neurochem 92, 519-24.
4. Sala, C. et al. (2001) Neuron 31, 115-30.
5. Boeckers, T.M. et al. (2002) J Neurochem 81, 903-10.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Human, Monkey, Bovine

Specificity / Sensitivity

SHANK2 Antibody recognizes endogenous levels of total SHANK2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val537 of human SHANK2 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The SHANK family proteins, also known as proline-rich synapse-associated proteins, consist of SHANK1, SHANK2, and SHANK3. SHANK proteins act as scaffolds at the neuronal post-synaptic density (PSD) (1), where they play a critical role in PSD assembly of excitatory synapses during development (2). While recruitment of SHANK proteins to the synapse is independent of their interaction with Homer (3), proper synaptic targeting of SHANK1 is mediated by interactions between its PDZ domain and PSD proteins (4). At the synapse, SHANK proteins interact with NMDA receptors and metabotropic glutamate receptor complexes (5). Research studies have proposed the involvement of SHANK proteins in autism and neurodegenerative diseases (6).

1. Grabrucker, A.M. et al. (2011) Trends Cell Biol 21, 594-603.
2. Boeckers, T.M. et al. (1999) J Neurosci 19, 6506-18.
3. Boeckers, T.M. et al. (2005) J Neurochem 92, 519-24.
4. Sala, C. et al. (2001) Neuron 31, 115-30.
5. Boeckers, T.M. et al. (2002) J Neurochem 81, 903-10.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.