CCR2 (D14H7) Rabbit mAb

• 产品编号：CST-12199S      品牌：CST       原厂货号：12199S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

此单克隆抗体通过用合成肽免疫动物制备，该合成肽是人CCR2蛋白Gly197周围的残基。CCR2是趋化因子G蛋白偶联受体的“CC分支”中的成员，能够调节单核细胞趋化性和T细胞迁移/活化以及在许多病理条件下能促进炎症反应（1）。CCR2是多种趋化因子的受体，包括MCP-1，MCP-3和MCP-4 (2-5)。CCR2能通过提高细胞内钙水平来进行信号转导。它有两个亚型，分别为CCR2A和CCR2B，两者羧基末端的尾巴有差异，CCR2B能够更有效地运输到膜（2,6）。CCR2最初是在THP-1单核细胞系中发现的，它的表达随着分化成巨噬细胞而减少（7）。敲除试验显示CCR2是巨噬细胞运输的主要“调节器”（8-10）。另外，研究表明，在一些HIV的感染中，CCR2与CD4是共同受体（11,12）。

1. Zhao, Q. (2010) J Leukoc Biol 88, 41-55.
2. Charo, I.F. et al. (1994) Proc Natl Acad Sci U S A 91, 2752-6.
3. Franci, C. et al. (1995) J Immunol 154, 6511-7.
4. Yamagami, S. et al. (1994) Biochem Biophys Res Commun 202, 1156-62.
5. Combadiere, C. et al. (1995) J Biol Chem 270, 29671-5.
6. Wong, L.M. et al. (1997) J Biol Chem 272, 1038-45.
7. Denholm, E.M. and Stankus, G.P. (1995) Cytokine 7, 436-40.
8. Kuziel, W.A. et al. (1997) Proc Natl Acad Sci U S A 94, 12053-8.
9. Boring, L. et al. (1997) J Clin Invest 100, 2552-61.
10. Kurihara, T. et al. (1997) J Exp Med 186, 1757-62.
11. Rucker, J. et al. (1996) Cell 87, 437-46.
12. Frade, J.M. et al. (1997) J Clin Invest 100, 497-502.

原厂资料：

Specificity / Sensitivity

CCR2 (D14H7) Rabbit mAb recognizes endogenous levels of total CCR2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly197 of human CCR2 protein.

Background

CCR2 is a member of the “CC-branch” of chemokine G protein-coupled receptors that regulate monocyte chemotaxis and T cell migration/activation and drive inflammation in a number of pathological conditions (1). CCR2 is the receptor for several chemokines including MCP-1, MCP-3, and MCP-4 (2-5). CCR2 transduces signals through increases in intracellular calcium levels. It has two alternative isoforms, CCR2A and CCR2B, differing in their carboxy-terminal tails with CCR2B trafficking more efficiently to the membrane (2,6). CCR2 was originally identified in the THP-1 monocyte cell line, and its expression is decreased following differentiation into macrophages (7). Knockout studies demonstrate that CCR2 is a major regulator of macrophage trafficking (8-10). In addition, research studies have shown that CCR2 functions as an alternative coreceptor with CD4 for infection of some strains of HIV (11,12).

1. Zhao, Q. (2010) J Leukoc Biol 88, 41-55.
2. Charo, I.F. et al. (1994) Proc Natl Acad Sci U S A 91, 2752-6.
3. Franci, C. et al. (1995) J Immunol 154, 6511-7.
4. Yamagami, S. et al. (1994) Biochem Biophys Res Commun 202, 1156-62.
5. Combadiere, C. et al. (1995) J Biol Chem 270, 29671-5.
6. Wong, L.M. et al. (1997) J Biol Chem 272, 1038-45.
7. Denholm, E.M. and Stankus, G.P. (1995) Cytokine 7, 436-40.
8. Kuziel, W.A. et al. (1997) Proc Natl Acad Sci U S A 94, 12053-8.
9. Boring, L. et al. (1997) J Clin Invest 100, 2552-61.
10. Kurihara, T. et al. (1997) J Exp Med 186, 1757-62.
11. Rucker, J. et al. (1996) Cell 87, 437-46.
12. Frade, J.M. et al. (1997) J Clin Invest 100, 497-502.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.