Bcl-11B (D6F1) XP? Rabbit mAb

• 产品编号：CST-12120S      品牌：CST       原厂货号：12120S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Bcl-11B (D6F1) XP®Rabbit mAb可以识别内源性的总Bcl-11B蛋白。合成对应人Bcl-11B蛋白邻近Leu391氨基酸残基的多肽免疫动物获得多克隆抗体。Bcl-11B (Ctip2)是一个COUP-TF作用蛋白，属于C2H2型锌指蛋白家族（1）。Bcl-11B在大脑中高表达，对于神经和其他组织和器官的发育是必须的。 Bcl-11B在T细胞系的功能和维护T细胞身份也扮演重要角色(1-3)。BCL11B 基因可能通过外显子的不同转录编码了BCL－11B的两种异构型蛋白。BCL－11B是一类通过其第4外显子(3)中第二和第三锌指结构域结合靶向基因的转录因子，同时也与包括COUP-TF proteins (1), the NuRD complex (5,6), HDAC1, HDAC2和 SUV39H1 (7)等多种蛋白伴侣相互作用。 已有研究发现Bcl-11B突变和丢失会导致小鼠的胸腺肿瘤的发生和人类的T细胞急性粒细胞白血病，显示其抑癌基因的角色(4,8)。研究机制发现Bcl-11B非依赖于P53 的抑制E3泛素连接酶HDM2的基因表达(9)。

1. Avram, D. et al. (2000) J Biol Chem 275, 10315-22.
2. Liu, P. et al. (2010) Immunol Rev 238, 138-49.
3. Kominami, R. (2012) Proc Jpn Acad Ser B Phys Biol Sci 88, 72-87.
4. Wakabayashi, Y. et al. (2003) Biochem Biophys Res Commun 301, 598-603.
5. Cismasiu, V.B. et al. (2005) Oncogene 24, 6753-64.
6. Topark-Ngarm, A. et al. (2006) J Biol Chem 281, 32272-83.
7. Marban, C. et al. (2007) EMBO J 26, 412-23.
8. Gutierrez, A. et al. (2011) Blood 118, 4169-73.
9. Obata, M. et al. (2012) Cell Signal 24, 1047-52.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey

Specificity / Sensitivity

Bcl-11B (D6F1) XP® Rabbit mAb recognizes endogenous levels of total Bcl-11B protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu391 of human Bcl-11B protein.

Background

Bcl-11B (Ctip2) is a COUP-TF interacting protein that belongs to the C2H2 type zinc finger protein family (1). Bcl-11B is highly expressed in the brain and is critical for the development of neurons, as well as other tissues and organs. Bcl-11B also plays an essential role in T cell lineage commitment and maintenance of T cell identity (1-3). Two isoforms of Bcl-11B are found to be encoded by the BCL11B gene, possibly through exon-skipping (4). Bcl-11B is a transcription factor which binds to target genes through the 2nd and 3rd zinc-finger domains of exon 4 (3), while also interacting with various protein partners including COUP-TF proteins (1), the NuRD complex (5,6), HDAC1, HDAC2, and SUV39H1 (7). Research studies have shown that mutations and deletion of Bcl-11B contribute to the development of thymic lymphoma in mice and T cell acute lymphoblastic leukemia in humans, indicating a role as a tumor suppressor (4,8). Mechanistic studies have shown that Bcl-11B represses gene expression of the E3 ubiquitin ligase HDM2 in a p53-dependent manner (9).

1. Avram, D. et al. (2000) J Biol Chem 275, 10315-22.
2. Liu, P. et al. (2010) Immunol Rev 238, 138-49.
3. Kominami, R. (2012) Proc Jpn Acad Ser B Phys Biol Sci 88, 72-87.
4. Wakabayashi, Y. et al. (2003) Biochem Biophys Res Commun 301, 598-603.
5. Cismasiu, V.B. et al. (2005) Oncogene 24, 6753-64.
6. Topark-Ngarm, A. et al. (2006) J Biol Chem 281, 32272-83.
7. Marban, C. et al. (2007) EMBO J 26, 412-23.
8. Gutierrez, A. et al. (2011) Blood 118, 4169-73.
9. Obata, M. et al. (2012) Cell Signal 24, 1047-52.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.