PGAM1 (D3J9T) Rabbit mAb

• 产品编号：CST-12098S      品牌：CST       原厂货号：12098S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PGAM1 (D3J9T) Rabbit mAb能够检测内源性PGAM1总蛋白水平。该单克隆抗体是由合成的人源PGAM1蛋白C末端肽段免疫动物制备的。磷酸甘油酸变位酶（PGAM1）在糖酵解过程中催化3-磷酸甘油酸转化成2-磷酸甘油酸（1-5）。研究表明，在发生癌症（1-4）和精神疾病（5）时，PGAM1会上调表达。尤其是在PKM2表达的细胞中，PGAM1的11位组氨酸位点会被磷酸烯醇式丙酮酸盐（PEP）磷酸化，这表明在活跃增殖的细胞中，PGAM1可能在通过一种替代的糖酵解途径发挥着调控作用(1)。

1. Vander Heiden, M.G. et al. (2010) Science 329, 1492-9.
2. Jacobowitz, D.M. et al. (2008) Microvasc Res 76, 89-93.
3. Ren, F. et al. (2010) Mol Cancer 9, 81.
4. Evans, M.J. et al. (2005) Nat Biotechnol 23, 1303-7.
5. Martins-de-Souza, D. et al. (2009) BMC Psychiatry 9, 17.

原厂资料：

Specificity / Sensitivity

PGAM1 (D3J9T) Rabbit mAb recognizes endogenous levels of total PGAM1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human PGAM1 protein.

Background

Phosphoglycerate mutase (PGAM1) catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate during glycolysis (1-5). Research studies have shown increased PGAM1 expression in cancer (1-4) and mental disease (5). Specifically, PGAM1 was shown to be phosphorylated at His11 by phosphoenolpyruvate (PEP) in PKM2-expressing cells, suggesting a possible regulatory role for PGAM1 in actively proliferating cells via an alternative glycolytic pathway (1).

1. Vander Heiden, M.G. et al. (2010) Science 329, 1492-9.
2. Jacobowitz, D.M. et al. (2008) Microvasc Res 76, 89-93.
3. Ren, F. et al. (2010) Mol Cancer 9, 81.
4. Evans, M.J. et al. (2005) Nat Biotechnol 23, 1303-7.
5. Martins-de-Souza, D. et al. (2009) BMC Psychiatry 9, 17.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting  1:1000