PANK4 (D8N2C) Rabbit mAb

• 产品编号：CST-12055S      品牌：CST       原厂货号：12055S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

PANK4 (D8N2C) Rabbit mAb能够检测内源性PANK4总蛋白水平。该单克隆抗体是由合成的人源PANK4蛋白天冬酰胺(373位)肽段免疫动物而制备的。泛酰酸激酶（PANK）是一种酶，负责催化辅酶A（CoA）的合成中的第一步（1-4）。人体有四个PANK基因（PANK1-4）（1-4）。 PANK4在体内广泛表达，在肌肉中具有较高的表达水平（1,2）。在大鼠骨骼肌葡萄糖负荷情况下PANK4表达升高（2）。有证据显示，在体外试验中，大鼠PANK4与丙酮酸激酶M2（PKM2）共定位（2）。 在β-细胞凋亡中，PANK4也会通过降低Pro-caspase-9的表达水平以起到一定的保护作用（3）。研究表明，PANK2基因突变与脑铁积累（NBIA）这种神经退行性疾病相关，该病的原名是蛋白球色素退变综合症（1,4），。在果蝇NBIA模型中hPANK4的表达会导致不孕不育的异常表现型（4）。

1. Zhou, B. et al. (2001) Nat Genet 28, 345-9.
2. Li, Y. et al. (2005) Mol Cell Biochem 277, 117-25.
3. Xiang, R.L. et al. (2007) Cell Res 17, 966-8.
4. Wu, Z. et al. (2009) Hum Mol Genet 18, 3659-72.

原厂资料：

pecificity / Sensitivity

PANK4 (D8N2C) Rabbit mAb recognizes endogenous levels of total PANK4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn373 of human PANK4 protein.

Background

Panthothenate kinase (PANK) is an enzyme that is responsible for catalyzing the first step in coenzyme A (CoA) synthesis (1-4). There are four human PANK genes (PANK1-4) (1-4). PANK4 is ubiquitously expressed with higher expression levels seen in muscle (1,2). PANK4 expression is elevated in rat skeletal muscle upon glucose challenge (2). There is evidence that rat PANK4 colocalizes with pyruvate kinase M2 (PKM2) in vitro (2). PANK4 may also play a protective role in beta-cell apoptosis by lowering the levels of pro-caspase-9 (3). Research studies have shown that mutations in the PANK2 gene are associated with Neurodegeneration with Brain Iron Accumulation (NBIA), formerly known as Hallervorden-Spatz syndrome (1,4). Expression of hPANK4 in a Drosophila model of NBIA rescues the phenotype with the exception of infertility (4).

1. Zhou, B. et al. (2001) Nat Genet 28, 345-9.
2. Li, Y. et al. (2005) Mol Cell Biochem 277, 117-25.
3. Xiang, R.L. et al. (2007) Cell Res 17, 966-8.
4. Wu, Z. et al. (2009) Hum Mol Genet 18, 3659-72.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.
Recommended Antibody Dilutions:
Western blotting  1:1000
Immunoprecipitation  1:50