Upf1 (D15G6) Rabbit mAb

• 产品编号：CST-12040S      品牌：CST       原厂货号：12040S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Upf1 (D15G6) Rabbit mAb 能够检测内源性的Upf1总蛋白。该单克隆抗体是经由合成的人Upf1蛋白的羧基端多肽免疫动物而生产的。Upf1蛋白被确认是无义介导的降解（NMD)中的一个活性组分，NMD是真核细胞中mRNA的一种监控机制，能够降解含有提前终止密码子的mRNA(1)。研究发现，Upf1蛋白是胞质中一个ATP依赖的RNA解旋酶(2)，后来发现它还是细胞质加工小体（cytoplasmic P-bodies）中的一个成分(3)。Upf1蛋白的磷酸化能够介导翻译抑制，其伴随着NMD作用，因而允许mRNA更易进入NMD机制(4)。此外，还鉴定出了NMD的另外两个活化分子，Upf2和Upf3。分别定位于核周以及核质(5)。

1. Leeds, P. et al. (1991) Genes Dev 5, 2303-14.
2. Weng, Y. et al. (1996) Mol Cell Biol 16, 5477-90.
3. Bruno, I. and Wilkinson, M.F. (2006) Cell 125, 1036-8.
4. Isken, O. et al. (2008) Cell 133, 314-27.
5. Lykke-Andersen, J. et al. (2000) Cell 103, 1121-31.

原厂资料：

Specificity / Sensitivity

Upf1 (D15G6) Rabbit mAb recognizes endogenous levels of total Upf1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Upf1 protein.

Background

Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5).

1. Leeds, P. et al. (1991) Genes Dev 5, 2303-14.
2. Weng, Y. et al. (1996) Mol Cell Biol 16, 5477-90.
3. Bruno, I. and Wilkinson, M.F. (2006) Cell 125, 1036-8.
4. Isken, O. et al. (2008) Cell 133, 314-27.
5. Lykke-Andersen, J. et al. (2000) Cell 103, 1121-31.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.