Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb

• 产品编号：CST-12028S      品牌：CST       原厂货号：12028S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

单克隆抗体由合成肽段免疫动物产生，该肽段与人cdc25C蛋白的48位苏氨酸邻近残基序列一致。Cdc25 是一种蛋白磷酸酯酶，能够去磷酸化激活cdc2，这是对于所有真核细胞进入有丝分裂期这关键一步的调控（1）。在有丝分裂间期，cdc25C的216位丝氨酸会一直被c-TAK1磷酸化，该位点的磷酸化在G2/M检验期依赖于DNA损伤（2）。当216位丝氨酸被磷酸化后，cdc25C可以和14-3-3家族蛋白结合，是cdc25C退回细胞质避免过早的有丝分裂（3）。检验点激酶Chk1和Chk2在DNA损伤后会磷酸化cdc25C的216位丝氨酸（4,5）。cdc25C的完全激活需要被cdc2/cyclin B和Polo-样激酶对其超过12个不同位点进行磷酸化，以及Pin1的活性，Pin1是一个肽脯氨酰异构酶 (PPI) (6,7). Pin1包括了1个WW结构域可以和磷酸化-丝氨酸/苏氨酸-脯氨酸位点结合以及一个催化PPI区域，该PPI区域可以诱导蛋白在磷酸化-丝氨酸/苏氨酸-脯氨酸位点形成顺/反异构体(8). cdc25C的48和67位苏氨酸被磷酸化后，cdc25C可以直接与Pin1的WW结构域结合（9）。48位苏氨酸磷酸化还会介导cdc25C与CKS/p13SUC1的结合 (10)。

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Monkey

Specificity / Sensitivity

Phospho-cdc25C (Thr48) (D2H3) Rabbit mAb recognizes endogenous levels of cdc25C protein only when phosphorylated at Thr48.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr48 of human cdc25C protein

Background

Cdc25 is a protein phosphatase responsible for dephosphorylating and activating cdc2, a crucial step in regulating the entry of all eukaryotic cells into mitosis (1). cdc25C is constitutively phosphorylated at Ser216 throughout interphase by c-TAK1, while phosphorylation at this site is DNA damage-dependent at the G2/M checkpoint (2). When phosphorylated at Ser216, cdc25C binds to members of the 14-3-3 family of proteins, sequestering cdc25C in the cytoplasm and thereby preventing premature mitosis (3). The checkpoint kinases Chk1 and Chk2 phosphorylate cdc25C at Ser216 in response to DNA damage (4,5).Full activation of cdc25C involves phosphorylation at more than 12 different sites by cdc2/cyclin B and Polo-like kinase, and the activity of Pin1, a peptidyl-prolyl isomerase (PPI) (6,7). Pin1 contains a WW domain that binds phospho-Ser/Thr-Pro sites and a catalytic PPI region that induces a cis/trans isomerization at phospho-Ser/Thr-Pro bonds (8). Thr48 and Thr67 of cdc25C interact directly with the WW domain of Pin1 when these sites are phosphorylated (9). Thr48 phosphorylation also mediates binding to CKS/p13SUC1 (10).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.