Phospho-PDGF Receptor α (Tyr762) Antibody

• 产品编号：CST-12022S      品牌：Cell Signaling Technology       原厂货号：12022S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

只有当Tyr762磷酸化时，PDGF Receptor α (Tyr762) Antibody能够识别内源性水平的PDGF 受体α蛋白。该抗体不与内源性水平的磷酸化PDGF 受体β蛋白发生交叉反应。该多克隆抗体是通过用合成磷酸肽免疫动物而制备的，该合成磷酸肽是人PDGF受体α蛋白Tyr762附近的残基。抗体由蛋白A和肽亲和层析法纯化。血小板衍生生长因子(PDGF) 家族的蛋白以几种二硫键连接的二聚体形式存在 (PDGF AA, PDGF AB, PDGF BB, PDGF CC和PDGF DD)，它们以特定的方式与两种密切相关的酪氨酸激酶受体-PDGF receptor α (PDGFRα)和PDGF receptor β (PDGFRβ)-结合。PDGFRα 和PDGFRβ 的胞内激酶区有75% 到85% 的序列同源性，而激酶插入区和羧基端仅有很低的同源性(27% 到 28%) (1). PDGFRα 同型二聚体和除了含有PDGF D 之外的所有的PDGF亚型结合。PDGFRβ同型二聚体能结合PDGF BB 和DD亚型，还有PDGF AB 异二聚体。 异聚体 PDGF receptor α/β能结合 PDGF B, C, 和 D 同型二聚体，还有 PDGF AB 异二聚体 (2)。 PDGFRα 和 PDGFRβ 都能被PDGF激活和 EGFR形成异二聚体, (3)。各种细胞的受体总数、受体亚基组成都不相同，因此不同细胞对PDGF结合的反应也不同(4)。配体结合能诱发受体的二聚化和自磷酸化，继而结合并激活胞内含有SH2结构的信号传导分子， 如Grb2, Src, GAP, PI3K激酶, PLCγ和 Nck。激活的PDGFRs能引发一系列不同的信号通路 并影响细胞的生长、肌动蛋白重塑、迁移和分化 (5)。

1. Deuel, T.F. et al. (1988) Biofactors 1, 213-7.
2. Bergsten, E. et al. (2001) Nat Cell Biol 3, 512-6.
3. Betsholtz, C. et al. (2001) Bioessays 23, 494-507.
4. Coughlin, S.R. et al. (1988) Prog Clin Biol Res 266, 39-45.
5. Ostman, A. and Heldin, C.H. (2001) Adv Cancer Res 80, 1-38.
6. Rikova, K. et al. (2007) Cell 131, 1190-203.
7. Matsumoto, T. et al. (2000) Biochem Biophys Res Commun 270, 28-33.
8. Yokote, K. et al. (1998) Oncogene 16, 1229-39.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-PDGF Receptor α (Tyr762) Antibody recognizes endogenous levels of PDGF receptor α protein only when phosphorylated at Tyr762. This antibody does not cross-react with endogenous levels of phosphorylated PDGF receptor β protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr762 of human PDGF receptor α protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Platelet derived growth factor (PDGF) family proteins exist as several disulphide-bonded, dimeric isoforms (PDGF AA, PDGF AB, PDGF BB, PDGF CC, and PDGF DD) that bind in a specific pattern to two closely related receptor tyrosine kinases, PDGF receptor α (PDGFRα) and PDGF receptor β (PDGFRβ). PDGFRα and PDGFRβ share 75% to 85% sequence homology between their two intracellular kinase domains, while the kinase insert and carboxy-terminal tail regions display a lower level (27% to 28%) of homology (1). PDGFRα homodimers bind all PDGF isoforms except those containing PDGF D. PDGFRβ homodimers bind PDGF BB and DD isoforms, as well as the PDGF AB heterodimer. The heteromeric PDGF receptor α/β binds PDGF B, C, and D homodimers, as well as the PDGF AB heterodimer (2). PDGFRα and PDGFRβ can each form heterodimers with EGFR, which is also activated by PDGF (3). Various cells differ in the total number of receptors present and in the receptor subunit composition, which may account for responsive differences among cell types to PDGF binding (4). Ligand binding induces receptor dimerization and autophosphorylation, followed by binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules, such as GRB2, Src, GAP, PI3 kinase, PLCγ, and NCK. A number of different signaling pathways are initiated by activated PDGF receptors and lead to control of cell growth, actin reorganization, migration, and differentiation (5).Phosphorylation of PDGFRα at Tyr762 was identified at Cell Signaling Technology using PTMScan®, our LC-MS/MS platform for phosphorylation site discovery (6). Tyr762 is located in the activation loop of the PDGFRα kinase domain. Phosphorylation of PDGFRα at this site was also reported by several other labs to be a docking site for CrkII and CrkL upon induction by growth factor treatment (7,8).PDGFRα中Tyr762的磷酸化是在Cell Signaling 

1. Deuel, T.F. et al. (1988) Biofactors 1, 213-7.
2. Bergsten, E. et al. (2001) Nat Cell Biol 3, 512-6.
3. Betsholtz, C. et al. (2001) Bioessays 23, 494-507.
4. Coughlin, S.R. et al. (1988) Prog Clin Biol Res 266, 39-45.
5. Ostman, A. and Heldin, C.H. (2001) Adv Cancer Res 80, 1-38.
6. Rikova, K. et al. (2007) Cell 131, 1190-203.
7. Matsumoto, T. et al. (2000) Biochem Biophys Res Commun 270, 28-33.
8. Yokote, K. et al. (1998) Oncogene 16, 1229-39.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody.