Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb

• 产品编号：CST-12005S      品牌：CST       原厂货号：12005S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb可以识别Ser709被磷酸化的REPS1蛋白。REPS1是一个含有RalBP1-associated EH同源结构域的蛋白。REPS1含有一个EH结构域，紧随其后的是2个富含脯氨酸的区域，以及一个C末端螺旋换结构域以结合RalBP1（1）。REPS1的EH结构域与Rab11-FIP2的NPF结构域结合，接到了他们共定位到核内体囊泡，影响EGFR内吞（2）。REPS1的两个富含脯氨酸的区域对于蛋白与GRK/GRB2的SH3结构域结合是非常重要的而且能够调控EGFR下游的信号通路。RESP1富含脯氨酸的区域也被认为可以和intersectin1 (ITSN1)的SH3结构域结合，引起网格蛋白小窝形成ITSN1/SGIP1/REPS1复合物（3）。REPS1发现了3中剪切异构体。Cell Signaling Technology PTMScan®技术，发现磷酸化修饰位点的LC-MS/MS平台，发现了REPS1的Ser709磷酸化。

1. Yamaguchi, A. et al. (1997) J Biol Chem 272, 31230-4.
2. Cullis, D.N. et al. (2002) J Biol Chem 277, 49158-66.
3. Dergai, O. et al. (2010) Biochem Biophys Res Commun 402, 408-13.
4. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

原厂资料：

Specificity / Sensitivity

Phospho-REPS1 (Ser709) (D8C1) Rabbit mAb recognizes endogenous levels of REPS1 protein only when phosphorylated at Ser709.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser709 of human REPS1 protein.

Background

REPS1 is a RalBP1-associated EH-homology domain containing protein. The sequence of REPS1 has an EH domain, followed by two proline-rich segments, and a C-terminal coiled-coil domain for binding to RalBP1 (1). The EH domain of REPS1 interacts with the NPF motif of Rab11-FIP2, mediates their colocalization to endosome vesicles, and influences EGFR endocytosis (2). The two proline-rich regions of REPS1 are important for binding to the SH3 domain of GRK/GRB2 and further regulate EGFR downstream signaling. The proline-rich regions of REPS1 have also been shown to interact with the SH3 domain of intersectin1 (ITSN1) and contribute to ITSN1/SGIP1/REPS1 complex formation on clathrin-coated pits (3). Three alternatively spliced isoforms of REPS1 have been identified.

Phosphorylation of REPS1 at Ser709 was identified at Cell Signaling Technology using PTMScan® Technology, our LC-MS/MS platform for phosphorylation site discovery (4).

Cell Signaling Technology PTMScan®技术，发现磷酸化修饰位点的LC-MS/MS平台，发现了REPS1的Ser709磷酸化。

1. Yamaguchi, A. et al. (1997) J Biol Chem 272, 31230-4.
2. Cullis, D.N. et al. (2002) J Biol Chem 277, 49158-66.
3. Dergai, O. et al. (2010) Biochem Biophys Res Commun 402, 408-13.
4. Rush, J. et al. (2005) Nat Biotechnol 23, 94-101.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody