PhosphoPlus? IGF-I Receptor β Antibody Duet

• 产品编号：CST-12004S      品牌：CST       原厂货号：12004S
• 产品分类：抗体 > 一抗 > 抗体对
• 应用分类：

其它组分：

描述：

CST的PhosphoPlus® Duets系列产品提出了一个评价蛋白质活性状态的方法。每个套装中都包含一支您感兴趣的蛋白活性状态抗体以及其总蛋白抗体。这些抗体是从CST各种特定应用的高性能抗体产品中优选的。I型胰岛素样生长因子受体（IGF-IR）是一种跨膜受体酪氨酸激酶，在胎儿和产后组织的多种细胞系中均有广泛表达（1-3）。受体与IGF-I和IGF-II配体结合后发生自磷酸化。激酶结构域的三个酪氨酸残基（Tyr1131，Tyr1135，Tyr1136）是最早期的三个主要磷酸化位点（4）。三个酪氨酸残基的磷酸化对于激酶的活化是必需的（5,6）。胰岛素受体（IRs）与IGF-I受体在结构和功能方面有着显著的相似性，包括它们激酶结构域激活化环中都有着相当于酪氨酸残基（Tyr1146/1150/1151）的存在。IRs的酪氨酸自磷酸化是细胞对于胰岛素刺激最早的应答反应之一（7）。自身磷酸化从Tyr1146和Tyr1150或Tyr1151位点的磷酸化开始，但完全的激酶活性需要三个酪氨酸残基的磷酸化（8）。

1. Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93.
2. Baserga, R. (2000) Oncogene 19, 5574-81.
3. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8.
4. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81.
5. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60.
6. Baserga, R. (1999) Exp Cell Res 253, 1-6.
7. White, M.F. et al. (1985) J Biol Chem 260, 9470-8.
8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80.

原厂资料：

Description

PhosphoPlus® Duets from Cell Signaling Technology (CST) provide a means to assess protein activation status. Each Duet contains an activation-state and total protein antibody to your target of interest. These antibodies have been selected from CST's product offering based upon superior performance in specified applications.

Background

Type I insulin-like growth factor receptor (IGF-IR) is a transmembrane receptor tyrosine kinase that is widely expressed in many cell lines and cell types within fetal and postnatal tissues (1-3). Receptor autophosphorylation follows binding of the IGF-I and IGF-II ligands. Three tyrosine residues within the kinase domain (Tyr1131, Tyr1135, and Tyr1136) are the earliest major autophosphorylation sites (4). Phosphorylation of these three tyrosine residues is necessary for kinase activation (5,6). Insulin receptors (IRs) share significant structural and functional similarity with IGF-I receptors, including the presence of an equivalent tyrosine cluster (Tyr1146/1150/1151) within the kinase domain activation loop. Tyrosine autophosphorylation of IRs is one of the earliest cellular responses to insulin stimulation (7). Autophosphorylation begins with phosphorylation at Tyr1146 and either Tyr1150 or Tyr1151, while full kinase activation requires triple tyrosine phosphorylation (8).

1. Adams, T.E. et al. (2000) Cell Mol Life Sci 57, 1050-93.
2. Baserga, R. (2000) Oncogene 19, 5574-81.
3. Scheidegger, K.J. et al. (2000) J Biol Chem 275, 38921-8.
4. Hernández-Sánchez, C. et al. (1995) J Biol Chem 270, 29176-81.
5. Lopaczynski, W. et al. (2000) Biochem Biophys Res Commun 279, 955-60.
6. Baserga, R. (1999) Exp Cell Res 253, 1-6.
7. White, M.F. et al. (1985) J Biol Chem 260, 9470-8.
8. White, M.F. et al. (1988) J Biol Chem 263, 2969-80.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150
mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02%
sodium azide. Store at –20°C. Do not aliquot the antibody.