ID3 (D16D10) Rabbit mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-11997S      品牌：CST       原厂货号：11997S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

ID3 (D16D10) Rabbit mAb兔单抗 (Alexa Fluor^® 488 偶联)可以识别内源性的总ID3蛋白.此单克隆抗体由合成肽段免疫动物产生，该肽段与人ID3蛋白羧基末端附近氨基酸残基序列一致。此Cell Signaling Technology公司抗体偶联Alexa Fluor^® 488 荧光染色并经人类细胞免疫荧光分析内部测试。抗体预期和未偶联的ID3 (D16D10) Rabbit mAb #9837显示同样的物种交叉反应.DNA-结合/分化蛋白抑制因子（ID）是一组抑制碱性螺旋-环-螺旋（bHLH）转录因子活性的蛋白。在人体中发现了4种ID蛋白（ID1-4），它们都含有一个螺旋-环-螺旋结构域，但缺乏碱性DNA结合结构域。与bHLH转录因子结合形成的异源二聚体可以减弱bHLH蛋白活性并阻止它们与DNA结合（1）。ID蛋白可以在发育过程中发挥功能，抑制干细胞/祖细胞提前成熟分化（1,2）。ID3在免疫系统发育过程中发挥了作用，可以抑制E2A介导的T细胞分化（3）。使用小鼠模型的研究发现纯和敲除ID3会损害T细胞分化（4），导致γδ T细胞淋巴瘤发生（5）。除了造血系统，ID3还会抑制MyoD，提示ID3在TGFβ介导的肌肉修复过程中有一定作用（6）。类似的，研究显示ID3会抑制结肠癌细胞中的p21，发挥促进潜在癌细胞自我更新的功能（7）。

1. Yokota, Y. (2001) Oncogene 20, 8290-8.
2. Hong, S.H. et al. (2011) J Cell Sci 124, 1445-52.
3. Miyazaki, M. et al. (2011) Nat Immunol 12, 992-1001.
4. Maruyama, T. et al. (2011) Nat Immunol 12, 86-95.
5. Li, J. et al. (2010) Blood 116, 5615-21.
6. Clever, J.L. et al. (2010) Am J Physiol Cell Physiol 298, C1087-99.
7. O'Brien, C.A. et al. (2012) Cancer Cell 21, 777-92.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog

Specificity / Sensitivity

ID3 (D16D10) Rabbit mAb (Alexa Fluor^® 488 Conjugate) recognizes endogenous levels of total ID3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human ID3 protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 488 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated ID3 (D16D10) Rabbit mAb #9837.

Background

Inhibitor of DNA-binding/Differentiation (ID) proteins are a family of proteins that function to repress the activity of basic helix-loop-helix (bHLH) transcription factors. There are four known ID proteins in humans (ID1-4), all of which contain a helix-loop-helix domain but lack a basic DNA binding domain. Heterodimerization with bHLH transcription factors therefore functions to sequester bHLH proteins and prevent their binding to DNA (1). ID proteins play important functional roles in development, primarily by inhibiting premature differentiation of stem/progenitor cells (1,2). ID3 plays an important role in immune system development where it has been shown to repress E2A-mediated differentiation of T cells (3). Studies in mouse models have shown that homozygous deletion of ID3 disrupts regulatory T cell differentiation (4) and leads to development of γδ T cell lymphoma (5). Outside of the hematopoietic compartment, ID3 was shown to repress MyoD, implicating ID3 in TGFβ-mediated muscle repair (6). Similarly, research studies have shown that ID3 suppresses p21 in colon cancer cells, a function that is purported to promote the self-renewal capacity of putative cancer-initiating cells (7).

1. Yokota, Y. (2001) Oncogene 20, 8290-8.
2. Hong, S.H. et al. (2011) J Cell Sci 124, 1445-52.
3. Miyazaki, M. et al. (2011) Nat Immunol 12, 992-1001.
4. Maruyama, T. et al. (2011) Nat Immunol 12, 86-95.
5. Li, J. et al. (2010) Blood 116, 5615-21.
6. Clever, J.L. et al. (2010) Am J Physiol Cell Physiol 298, C1087-99.
7. O'Brien, C.A. et al. (2012) Cancer Cell 21, 777-92.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium
azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the
antibodies. Protect from light. Do not freeze.
*Species cross-reactivity is determined by western
blot using the unconjugated antibody.