Phospho-DDR1 (Tyr792) Antibody

• 产品编号：CST-11994S      品牌：CST       原厂货号：11994S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

只有当Tyr792磷酸化时，磷酸化DDR1 (Tyr792)抗体能够识别内源性水平的DDR1蛋白。该抗体可能与其他酪氨酸磷酸化RTKs发生交叉反应。该多克隆抗体是通过用合成磷酸肽免疫动物而生产的，该合成磷酸肽是人DDR1蛋白Tyr792附近的残基。抗体由蛋白A和肽亲和层析法纯化。盘状结构域受体(DDRs)是一种酪氨酸激酶受体，它的胞外区有一个盘状结构同源性重复，能够被与之相结合的胞外基质胶原激活。目前已知的哺乳动物的DDRs有两种：DDR1 和DDR2 (1)。它们广泛表达于人的组织中，并有可能与平滑肌细胞介导的胶原重塑相关（2）。DDRs异常表达并激活的下游通路可能与很多和基质降解和重构增加相关的人类疾病，如心血管疾病、肝纤维化和肿瘤侵袭性疾病密切相关（1）。盘状结构域受体(DDRs)是一种酪氨酸激酶受体，它的胞外区有一个盘状结构同源性重复，能够被与之相结合的胞外基质胶原激活。目前已知的哺乳动物的DDRs有两种：DDR1 和DDR2 (1)。它们广泛表达于人的组织中，并有可能与平滑肌细胞介导的胶原重塑相关（2）。DDRs异常表达并激活的下游通路可能与很多和基质降解和重构增加相关的人类疾病，如心血管疾病、肝纤维化和肿瘤侵袭性疾病密切相关（1）。

1. Vogel, W. (1999) FASEB J 13 Suppl, S77-82.
2. Ferri, N. et al. (2004) Am J Pathol 164, 1575-85.
3. Rikova, K. et al. (2007) Cell 131, 1190-203.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat

Specificity / Sensitivity

Phospho-DDR1 (Tyr792) Antibody recognizes endogenous levels of DDR1 protein only when phosphorylated at Tyr792. This antibody may cross-reacts with other tyrosine-phosphorylated RTKs.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr792 of human DDR1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

The discoidin domain receptors (DDRs) are receptor tyrosine kinases with a discoidin homology repeat in their extracellular domains, activated by binding to extracellular matrix collagens. So far, two mammalian DDRs have been identified: DDR1 and DDR2 (1). They are widely expressed in human tissues and may have roles in smooth muscle cell-mediated collagen remodeling (2). Research studies have implicated aberrant expression and signaling of DDRs in human diseases related to increased matrix degradation and remodeling, such as cardiovascular disease, liver fibrosis, and tumor invasion (1).Phosphorylation of DDR1 at Tyr792 was identified at Cell Signaling Technology using PTMScan®, our LC-MS/MS platform for phosphorylation site discovery (3). Tyr792 is located in the activation loop of the DDR1 kinase domain.

1. Vogel, W. (1999) FASEB J 13 Suppl, S77-82.
2. Ferri, N. et al. (2004) Am J Pathol 164, 1575-85.
3. Rikova, K. et al. (2007) Cell 131, 1190-203.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM
NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C.
Do not aliquot the antibody.