OTX2 (D7Y3J) Rabbit mAb

• 产品编号：CST-11943S      品牌：CST       原厂货号：11943S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

OTX2 (D7Y3J)Rabbit mAb可以识别内源性的总OTX2蛋白。此抗体与人OTX2蛋白的两个亚型都可以发生反应。此单克隆抗体由合成肽段免疫动物产生，该肽段与人OTX2蛋白Asn251邻近氨基酸残基序列一致。Orthodenticle homeobox 2 (OTX2)属于配对盒，含有同源结构域的转录因子bicoid超家族。OTX2是一个关键的重要神经元转录因子，参与调控神经元祖细胞中的细胞增殖和分化等细胞周期基因的表达（1-3）。除了在神经元发育过程中发挥作用，OTX2作用于非细胞自主的方式促进受损的视网膜神经节细胞的存活（4）。OTX2也被发现影响了有丝分裂后期神经元对有毒物质或生理应激的反应性（3）。值得注意的是，OTX2的异常表达与神经瘤发育密切相关。例如，研究发现OTX2在多种髓母细胞瘤细胞系中高表达，并且在一组原始髓母细胞瘤中同时发现了OTX2的高表达和基因扩增（5）。体外实验表明，OTX2表达异常直接影响髓母细胞瘤细胞系的扩增和衰老（6,7）。

1. Simeone, A. et al. (1993) EMBO J 12, 2735-47.
2. Omodei, D. et al. (2008) Development 135, 3459-70.
3. Chung, C.Y. et al. (2010) Brain 133, 2022-31.
4. Torero Ibad, R. et al. (2011) J Neurosci 31, 5495-503.
5. Adamson, D.C. et al. (2010) Cancer Res 70, 181-91.
6. Bunt, J. et al. (2011) Int J Cancer 131, E21-32.
7. Bunt, J. et al. (2010) Mol Cancer Res 8, 1344-57.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Mouse, Rat, Monkey, Chicken, Bovine, Guinea Pig, Horse

Specificity / Sensitivity

OTX2 (D7Y3J) Rabbit mAb recognizes endogenous levels of total OTX2 protein. This antibody recognizes both known human isoforms.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn251 of human OTX2 protein.

Background

Orthodenticle homeobox 2 (OTX2) belongs to the bicoid subfamily of paired-box, homeodomain-containing transcription factors. OTX2 is a critically important neuronal transcription factor that functions to regulate the expression of cell cycle genes controlling proliferation and differentiation of neural progenitor cells (1-3). In addition to its neuronal development functions, it has been reported that OTX2 can function in a non-cell autonomous manner to promote survival of damaged retinal ganglion cells (4). OTX2 has also been shown to influence the susceptibility of post-mitotic neurons to toxic insult or physiological stress (3). Notably, aberrant expression of OTX2 has been strongly linked with neuronal tumor development. For example, research studies have found OTX2 is overexpressed in many medulloblastoma cell lines, and both overexpression and gene amplification were reported in a subset of primary medulloblastomas (5). In vitro studies support these observations, as targeted alterations in OTX2 expression directly affected both proliferation and senescence of medulloblastoma cell lines (6,7).

1. Simeone, A. et al. (1993) EMBO J 12, 2735-47.
2. Omodei, D. et al. (2008) Development 135, 3459-70.
3. Chung, C.Y. et al. (2010) Brain 133, 2022-31.
4. Torero Ibad, R. et al. (2011) J Neurosci 31, 5495-503.
5. Adamson, D.C. et al. (2010) Cancer Res 70, 181-91.
6. Bunt, J. et al. (2011) Int J Cancer 131, E21-32.
7. Bunt, J. et al. (2010) Mol Cancer Res 8, 1344-57.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000