Oligophrenin-1 Antibody

• 产品编号：CST-11939S      品牌：CST       原厂货号：11939S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

Oligophrenin-1 Antibody 能够检测内源性的CPT1A总蛋白。该多克隆抗体是由合成的人源HMOX2/HO-2蛋白天冬氨酸(647位)肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。oligophrenin-1是一个RhoGTP酶激活蛋白，由OPHN1基因编码（1）。oligophrenin-1由一个N-末端BAR结构域，一个PH结构域，一个中央RhoGAP结构域和三个推测的C-末端SH3-结合位点组成。Oligophrenin-1在膜信号通路中起到了重要作用，这是通过其BAR结构域与弧形膜的相互作用， PH结构域与膜磷脂酰肌醇膜的结合以及SH3结合位点与接头蛋白的结合来实现的（1-3）。 oligophrenin-1调节突触小泡内吞作用（3），并在树突棘形态发生中起着重要的作用（4）。此外，通过与转录因子Rev-erbα相互作用并保护其免于降解，Oligophrenin-1参与调节了海马的昼夜节律（5）。研究表明X连锁智力障碍与Oligophrenin-1相关（1）。

1. Billuart, P. et al. (1998) Nature 392, 923-6.
2. Khelfaoui, M. et al. (2007) J Neurosci 27, 9439-50.
3. Nakano-Kobayashi, A. et al. (2009) Curr Biol 19, 1133-9.
4. Govek, E.E. et al. (2004) Nat Neurosci 7, 364-72.
5. Valnegri, P. et al. (2011) Nat Neurosci 14, 1293-301.

原厂资料：

Specificity / Sensitivity

Oligophrenin-1 Antibody recognizes endogenous levels of total Oligophrenin-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp647 of human Oligophrenin-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Oligophrenin-1 is a RhoGTPase-activating protein encoded by the gene OPHN1 (1). Oligophrenin-1 is composed of an N-terminal BAR domain, a pleckstrin homology domain, a central RhoGAP domain, and three putative C-terminal SH3-binding sites. Oligophrenin-1 plays a role in membrane signaling through interaction of its BAR domain with curved membranes, binding of its pleckstrin homology domain with membrane phosphoinositides, and interaction of the SH3-binding sites with adaptor proteins (1-3). Oligophrenin-1 regulates synaptic vesicle endocytosis (3) and plays an important role in dendritic spine morphogenesis (4). Furthermore, by interacting with the transcription factor Rev-erbα and protecting it from degradation, Oligophrenin-1 participates in the regulation of the circadian rhythm in the hippocampus (5). Research studies have demonstrated an involvement of Oligophrenin-1 in X-linked mental retardation (1).

1. Billuart, P. et al. (1998) Nature 392, 923-6.
2. Khelfaoui, M. et al. (2007) J Neurosci 27, 9439-50.
3. Nakano-Kobayashi, A. et al. (2009) Curr Biol 19, 1133-9.
4. Govek, E.E. et al. (2004) Nat Neurosci 7, 364-72.
5. Valnegri, P. et al. (2011) Nat Neurosci 14, 1293-301.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000