CAD Antibody

• 产品编号：CST-11933S      品牌：CST       原厂货号：11933S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CAD Antibody能够检测内源性CAD总蛋白水平。在一些细胞系中该抗体可能会某未知来源~45 kDa蛋白产生交叉反应。该多克隆抗体是由合成的人源CAD蛋白C末端肽段免疫动物而制备的。抗体由protein A和肽亲和层析技术纯化。

CAD蛋白对于嘧啶核苷酸的从头合成过程必不可少，它具有下列酶的活性：谷氨酰胺酰胺转移酶，氨甲酰磷酸合成酶，天冬氨酸转氨甲酰酶和二氢乳清酸酶。因此，CAD能够将谷氨酰胺转换为尿苷酸，后者是所有嘧啶碱基的一个共同前体，它对于核酸的合成也是必需的（1）。在静息细胞中，CAD主要定位于细胞质中负责合成嘧啶。当增殖细胞进入S期，MAP激酶（ERK1 / 2）催化CAD蛋白Thr456位磷酸化，使CAD易位到细胞核。当细胞退出S期，CAD 的Thr456位去磷酸化，Ser1406位在PKA作用下磷酸化，返回通路恢复基础活性（2）。各种研究表明CAD在几种类型的癌症中表达增加，促进了药理抑制剂如PALA的开发。进一步的研究已经将CAD确定为一个潜在的预测早期前列腺癌的复发标记（3）。

1. Coleman, P.F. et al. (1977) J Biol Chem 252, 6379-85.
2. Sigoillot, F.D. et al. (2005) J Biol Chem 280, 25611-20.
3. Morin, A. et al. (2012) FASEB J 26, 460-7.

原厂资料：

Specificity / Sensitivity

CAD Antibody recognizes endogenous levels of total CAD protein. This antibody may cross-react with a protein of unknown origin at ~45 kDa in some cell lines.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CAD protein. Antibodies are purified by protein A and peptide affinity chromatography.

Background

CAD is essential for the de novo synthesis of pyrimidine nucleotides and possesses the following enzymatic activities: glutamine amidotransferase, carbamoyl-phosphate synthetase, aspartate transcarbamoylase, and dihydroorotase. Thus, the enzyme converts glutamine to uridine monophosphate, a common precursor of all pyrimidine bases, and it is necessary for nucleic acid synthesis (1). In resting cells, CAD is localized mainly in the cytoplasm where it carries out pyrimidine synthesis. As proliferating cells enter S phase, MAP Kinase (Erk1/2) phosphorlyates CAD at Thr456, resulting in CAD translocation to the nucleus. As cells exit S phase, CAD is dephosphorylated at Thr456 and phosphorylated at Ser1406 by PKA, returning the pathway to basal activity (2). Various research studies have shown increased expression of CAD in several types of cancer, prompting the development of pharmacological inhibitors such as PALA. Further studies have identified CAD as a potential predictive early marker of prostate cancer relapse (3).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to
detect this antibody. Recommended Antibody Dilutions:
 Western blotting 1:1000