IKKα (3G12) Mouse mAb

• 产品编号：CST-11930S      品牌：CST       原厂货号：11930S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

IKKα (3G12) Mouse mAb鼠单抗能够检测内源性IKKα总蛋白水平。此单克隆抗体通过重组蛋白免疫动物制备，这种重组蛋白是人源IKKα蛋白上的一段特异性的片段。

NF-κB/Rel转录调控因子在细胞质中与IκB抑制蛋白结合以非活性形式存在(1-3)。大部分因子通过一个经典的信号通路，即磷酸化诱导、蛋白酶体介导降解IκB的方法激活NF-κB (3-7)。此通路中最重要的步骤是激活高分子量的IκB激酶（IKK）复合体，此复合体催化的反应中有三个紧密相关的IKK亚基参与完成，IKKα and IKKβ 作为激酶的催化单元，IKKγ作为调控单元(8,9)。IKK的激活依赖于特定位点的磷酸化：IKKβ活性环中的 Ser177和Ser181位点（IKKα中的ser176和ser180 位点），这些特异性的位点的磷酸化导致蛋白构象的变化从而激活激酶（10-13）。

原厂资料：

Specificity / Sensitivity

IKKα (3G12) Mouse mAb recognizes endogenous levels of total IKKα protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to a fragment of human IKKα protein.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation (10-13).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-mouse secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000
Immunofluorescence (IF-IC) 1:1600
Flow Cytometry 1:800