CHD4 (D8B12) Rabbit mAb

• 产品编号：CST-11912S      品牌：CST       原厂货号：11912S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

CHD4 (D8B12) Rabbit mAb兔单抗能够识别内源性CHD4总蛋白水平。基于序列信息分析，预测该抗体不与其他CHD蛋白发生交叉反应。

CHD4 (D8B12) Rabbit mAb兔单抗能够识别内源性CHD4总蛋白水平。基于序列信息分析，预测该抗体不与其他CHD蛋白发生交叉反应。

1. Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76.
2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
3. Tong, J.K. et al. (1998) Nature 395, 917-21.
4. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
5. Zhang, Y. et al. (1998) Cell 95, 279-89.
6. Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
7. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
8. Fujita, N. et al. (2003) Cell 113, 207-19.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Bovine, Pig, Guinea Pig, Horse

Specificity / Sensitivity

CHD4 (D8B12) Rabbit mAb recognizes endogenous levels of total CHD4 protein. Based on sequence alignment, this antibody is not predicted to cross-react with other CHD proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly1391 of human CHD4 protein.

Background

Chromodomain-helicase-DNA-binding domain (CHD) proteins have been identified in a variety of organisms (1,2). This family of nine proteins is divided into three separate subfamilies: subfamily I (CHD1 and CHD2), subfamily II (CHD3 and CHD4), and subfamily III (CHD5, CHD6, CHD7, CHD8, CHD9). All CHD proteins contain two tandem amino-terminal chromodomains, a SWI/SNF-related ATPase domain, and a carboxy-terminal DNA-binding domain (1,2). The chromodomains facilitate binding to methylated lysine residues of histone proteins and confer interactions with specific regions of chromatin. The SWI/SNF-related ATPase domain utilizes energy from ATP hydrolysis to modify chromatin structure. CHD proteins are often found in large, multiprotein complexes with their transcriptional activation or repression activity governed by other proteins within the complex. CHD3 (also known as Mi2-α) and CHD4 (also known as Mi2-β) are central components of the nucleosome remodeling and histone deacetylase (NuRD) transcriptional repressor complex, which also contains HDAC1, HDAC2, RBAP48, RBAP46, MTA1, MTA2, MTA3, and MBD3 (3-8). Both CHD3 and CHD4 contain two plant homeodomain (PHD) zinc finger domains that bind directly to HDAC1 and HDAC2.

1. Hall, J.A. and Georgel, P.T. (2007) Biochem Cell Biol 85, 463-76.
2. Marfella, C.G. and Imbalzano, A.N. (2007) Mutat Res 618, 30-40.
3. Tong, J.K. et al. (1998) Nature 395, 917-21.
4. Xue, Y. et al. (1998) Mol Cell 2, 851-61.
5. Zhang, Y. et al. (1998) Cell 95, 279-89.
6. Bowen, N.J. et al. (2004) Biochim Biophys Acta 1677, 52-7.
7. Jones, P.L. et al. (1998) Nat Genet 19, 187-91.
8. Fujita, N. et al. (2003) Cell 113, 207-19.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000
Immunoprecipitation 1:50