MDH2 (D8Q5S) Rabbit mAb

• 产品编号：CST-11908S      品牌：CST       原厂货号：11908S
• 产品分类：抗体 > 一抗 > 蛋白特异性一抗
• 应用分类：

描述：

MDH2 (D8Q5S) Rabbit mAb能够检测内源性MDH2总蛋白水平。该单克隆抗体是由合成的人源MDH2蛋白甘氨酸(306位)肽段免疫动物而制备的。苹果酸脱氢酶(MDH)是三羧酸循环和苹果酸/天冬氨酸穿梭过程中的关键酶(1,2)。MDH广泛表达于大多数细菌和所有的真核生物 (2)。细胞质MDH同工酶(cMDH或MDH1)主要是在苹果酸/天冬氨酸穿梭过程中将草酰乙酸还原成苹果酸(1,2,3)。而线粒体MDH同工酶(mMDH或MDH2)的作用主要是在三羧酸循环中将苹果酸氧化为草酰乙酸(1,2)。

1. Matsuda, T. et al. (2010) J Pharmacol Sci 112, 320-6.
2. Minárik, P. et al. (2002) Gen Physiol Biophys 21, 257-65.
3. Joh, T. et al. (1987) J Biol Chem 262, 15127-31.

原厂资料：

Specificity / Sensitivity

MDH2 (D8Q5S) Rabbit mAb recognizes endogenous levels of total MDH2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly306 of human MDH2 protein.

Background

Malate dehydrogenase (MDH) is a key enzyme in the tricarboxylic acid cycle and malate/aspartate shuttle (1,2). MDH is widely expressed in organisms from most bacteria to all eukaryotes (2). The cytoplasmic MDH isoenzyme (cMDH or MDH1) primarily reduces oxaloacetate to malate in the malate/aspartate shuttle (1-3). The major function of the mitochondrial MDH isoenzyme (mMDH or MDH2) is to oxidize malate to oxaloacetate in the tricarboxylic acid cycle (1,2).

1. Matsuda, T. et al. (2010) J Pharmacol Sci 112, 320-6.
2. Minárik, P. et al. (2002) Gen Physiol Biophys 21, 257-65.
3. Joh, T. et al. (1987) J Biol Chem 262, 15127-31.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western blotting 1:1000