Resazurin Cell Viability Kit

• 产品编号：CST-11884S      品牌：CST       原厂货号：11884S
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞增殖及周期检测试剂盒
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描述：

刃天青（Resazurin）细胞活性试剂盒能够检测活细胞中代谢酶转换刃天青产生的试卤灵。该试剂盒预计能在大多数细胞株中使用。对于大多数实验中，0.02-2×10⁵个细胞/孔应该是足够的，但也取决于细胞类型和孵育时间。为获得最佳结果，推荐进行细胞数滴定和孵育时间进程分析（图1中所示）。注：微生物污染物将减少刃天青转化为试卤灵，从而产生假阳性结果。自体荧光化合物可能会对这种测定方法有干扰。

刃天青（Resazurin）细胞活性试剂盒是荧光检测法，检测细胞的代谢活性。通过活细胞代谢中的脱氢酶，蓝色无荧光刃天青试剂被还原为高度荧光类试卤灵。这种转换只发生在活细胞中，因此，试卤灵的产生量与样品中的活细胞数成正比。检测中形成的试卤灵，可以用荧光光度计（Ex=530-570 nm, Em=590-620 nm）的相对荧光单位（RFU）来测量并量化。

细胞活性检测在研究生长因子、细胞因子和细胞毒性剂的药物开发中被广泛使用。无论是在早期的药物发现化合物筛选，还是随后的药物安全性和毒性研究中，高通量筛选都需要可靠、灵敏、简单的分析方法，能够分析大量的样品。采用四唑盐比色法细胞活力检测，如MTT、XTT和WST-1，都是基于活细胞能够还原四唑盐成高度着色的甲瓒化合物而开发的（1,2）。同样，在活细胞中，刃天青（蓝色和非荧光）可以变成试卤灵（粉红色和高荧光），因此可用来评估哺乳动物细胞毒性、生存能力、迁移和侵袭（3-6）。与XTT Cell Viability Kit #9095相似，刃天青细胞活性试剂盒不需要放射性材料、细胞固定或细胞通透性作用，而且，该测定中使用的细胞可用于进一步的分析。

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Specificity / Sensitivity

The Resazurin Cell Viability Kit detects resorufin produced from resazurin conversion by metabolic enzymes in live cells. This kit is expected to work in most cell lines. For most experiments, 0.02-2x10⁵ cells/well should be sufficient, but this can vary depending on the cell type and incubation time. For best results, a cell number titration and incubation time course (as shown in Figure 1) is recommended. 
 Note: Microbial contaminants will reduce resazurin to resorufin, yielding false positive results. Autofluorescent compounds may interfere with this assay.

Description

The Resazurin Cell Viability Kit is a fluorescent assay that detects cellular metabolic activity. The blue nonfluorescent resazurin reagent is reduced to highly fluorescent resorufin by dehydrogenase enzymes in metabolically active cells. This conversion only occurs in viable cells and thus, the amount of resorufin produced is proportional to the number of viable cells in the sample. The resorufin formed in the assay can be quantified by measuring the relative fluorescence units (RFU) using a fluorometer (Ex=530-570 nm, Em=590-620 nm).

Background

Cell viability assays are widely used in drug discovery for the study of growth factors, cytokines, and cytotoxic agents. High throughput screening, in both early drug discovery compound screening and subsequent drug safety and toxicity studies, require reliable, sensitive, and simple assays with the ability to analyze a large number of samples. Colorimetric cell viability assays using tetrazolium salt, such as MTT, XTT, and WST-1, were developed based on live cell reduction of tetrazolium salt into highly colored formazan compounds (1,2). Similarly, resazurin (blue and nonfluorescent) can be reduced to resorufin (pink and highly fluorescent) in live cells and is therefore used to assess mammalian cell toxicity, viability, migration, and invasion (3-6). Similar to the XTT Cell Viability Kit #9095, the Resazurin Cell Viability Kit does not require radioactive materials, cell fixation, or cell permeabilization, and cells used in this assay may be used for further analysis.

注意事项：

Note: Resazurin solution should be stored at –20°C in the dark for long term storage. It can be stored at 4°C in the dark for up to 12 months.