p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor? 594 Conjugate)

• 产品编号：CST-11850S      品牌：CST       原厂货号：11850S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

（偶联Alexa Fluor® 594）可以识别内源性总的p21蛋白。抗体与其他的CDK抑制因子没有交叉反应。单克隆抗体由合成肽段免疫动物产生，该肽段与人p21蛋白的羧基端一致。Cell Signaling Technology公司抗体偶联Alexa Fluor® 594荧光素染料并经人类细胞直接流式细胞仪免疫荧光分析内部测试。抗体预期和未偶联p21 Waf1/Cip1 (12D1)Rabbit mAb#2947显示同样的物种交叉反应.

肿瘤抑制蛋白p21 Waf1/Cip1可以作为细胞周期的抑制因子。它可以与细胞周期蛋白和细胞周期蛋白依赖性激酶形成异源三聚复合物。当和CDK2形成复合物后，它可以发回抑制及酶活性并中止细胞通过G1/S（1）。但是p21也可以促进CDK4或CDK6和细胞周期蛋白复合物的组合及功能（2）。p21的羧基端足以结合并抑制PCNA，DNA聚合酶的一个亚基，同时可能参与了细胞周期进程中的DNA复制（3）。当受到了UV损伤或处于cdc2/cyclin B 或 CDK2/cyclin A激活的细胞周期时，p53将被磷酸化并通过p53-反应元件上调p21表达（4）。p21蛋白通过泛素化和蛋白酶体降解（5）。 

1. Pestell, R.G. et al. (1999) Endocrine Rev. 20, 501-534.
2. Cheng, J. et al. (1999) EMBO J. 18, 1571-1583.
3. Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659.
4. Wang, Y. and Prives, C. (1995) Nature 376, 88-91.
5. Sheaff, R.J. et al. (2000) Cell 5, 403-410.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Dog

Specificity / Sensitivity

p21 Waf1/Cip1 (12D1) Rabbit mAb (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of total p21 protein. The antibody does not cross-react with other CDK inhibitors.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human p21 protein.

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 594 fluorescent dye and tested in-house for direct immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated p21 Waf1/Cip1 (12D1) Rabbit mAb #2947.

Background

The tumor suppressor protein p21 Waf1/Cip1 acts as an inhibitor of cell cycle progression. It functions in stoichiometric relationships forming heterotrimeric complexes with cyclins and cyclin-dependent kinases. In association with CDK2 complexes, it serves to inhibit kinase activity and block progression through G1/S (1). However, p21 may also enhance assembly and activity in complexes of CDK4 or CDK6 and cyclin D (2). The carboxy-terminal region of p21 is sufficient to bind and inhibit PCNA, a subunit of DNA polymerase, and may coordinate DNA replication with cell cycle progression (3). Upon UV damage or during cell cycle stages when cdc2/cyclin B or CDK2/cyclin A are active, p53 is phosphorylated and upregulates p21 transcription via a p53-responsive element (4). Protein levels of p21 are downregulated through ubiquitination and proteasomal degradation (5).

1. Pestell, R.G. et al. (1999) Endocrine Rev. 20, 501-534.
2. Cheng, J. et al. (1999) EMBO J. 18, 1571-1583.
3. Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659.
4. Wang, Y. and Prives, C. (1995) Nature 376, 88-91.
5. Sheaff, R.J. et al. (2000) Cell 5, 403-410.

注意事项：

Storage: Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C

Recommended Antibody Dilutions:
Immunofluorescence (IF-IC) 1:50