PAX5 (D19F8) XP? Rabbit mAb (Alexa Fluor? 488 Conjugate)

• 产品编号：CST-11849S      品牌：CST       原厂货号：11849S
• 产品分类：抗体 > 一抗 > 染料标记抗体
• 应用分类：

描述：

PAX5 (D19F8) XP^® Rabbit mAb 兔单抗(Alexa Fluor^® 488 偶联)可以识别内源性的总PAX5蛋白。此单克隆抗体由合成肽段免疫动物产生，该肽段与人PAX5蛋白Gln350邻近氨基酸残基序列一致。此 Cell Signaling Technology公司抗体偶联Alexa Fluor^® 488 荧光染色，并经人类细胞免疫荧光分析进行内部测试。抗体预期和未偶联的PAX5 (D19F8) XP^® Rabbit mAb兔单抗 #8970显示同样的物种交叉反应。Paired box (PAX)蛋白是一组在动物发育过程中发挥了重要的及多样性作用的转录因子家族（1）。人和其他哺乳动物中发现了9个PAX蛋白（PAX1-9）。它们都含有氨基端“配对”结构域，包括了2个具有DNA结合活性的螺旋-转角-螺旋模体（2）。PAX蛋白，基于一个羧基端同源域和中心八肽区域的存在与否，可以根据不同的结构分为4个不同的亚型(I-IV)。亚型I (PAX1 和9)包括八肽但是缺乏同源域；亚型II (PAX2, 5, 和 8)包括了八肽和被截短的同源域；亚型III (PAX3 和7)包括了八肽和完整的同源域；和亚型IV (PAX4 和6)包括了一个完整的同源域但是缺乏八肽结构（2）。PAX蛋白在发育过程中发挥了重要作用调控了胚胎模式和器官发生过程中的转录调控网络（3）；PAX蛋白的一组亚型还在产后发育过程中发挥重要功能（4）。研究提示基因突变引发的PAX基因表达异常与多种癌症相关（1-3），部分亚型II和III被认为是癌症进程中可能的介导因子（2）。

1. Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
2. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
3. Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
4. Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.

原厂资料：

Homology

Species predicted to react based on 100% sequence homology: Xenopus

Specificity / Sensitivity

 PAX5 (D19F8) XP^® Rabbit mAb (Alexa Fluor^® 488 Conjugate) recognizes endogenous levels of total PAX5 protein.

Source / Purification

 Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln350 of human PAX5 protein.

Description

 This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 488 fluorescent dye and tested in-house for immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated PAX5 (D19F8) XP^® Rabbit mAb #8970.

Background

 Paired box (PAX) proteins are a family of transcription factors that play important and diverse roles in animal development (1). Nine PAX proteins (PAX1-9) have been described in humans and other mammals. They are defined by the presence of an amino-terminal "paired" domain, consisting of two helix-turn-helix motifs, with DNA binding activity (2). PAX proteins are classified into four structurally distinct subgroups (I-IV) based on the absence or presence of a carboxy-terminal homeodomain and a central octapeptide region. Subgroup I (PAX1 and 9) contains the octapeptide but lacks the homeodomain; subgroup II (PAX2, 5, and 8) contains the octapeptide and a truncated homeodomain; subgroup III (PAX3 and 7) contains the octapeptide and a complete homeodomain; and subgroup IV (PAX4 and 6) contains a complete homeodomain but lacks the octapeptide region (2). PAX proteins play critically important roles in development by regulating transcriptional networks responsible for embryonic patterning and organogenesis (3); a subset of PAX proteins also maintain functional importance during postnatal development (4). Research studies have implicated genetic mutations that result in aberrant expression of PAX genes in a number of cancer subtypes (1-3), with members of subgroups II and III identified as potential mediators of tumor progression (2).

1. Lang, D. et al. (2007) Biochem Pharmacol 73, 1-14.
2. Robson, E.J. et al. (2006) Nat Rev Cancer 6, 52-62.
3. Wang, Q. et al. (2008) J Cell Mol Med 12, 2281-94.
4. Blake, J.A. et al. (2008) Dev Dyn 237, 2791-803.