HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate)

• 产品编号：CST-11846S      品牌：CST       原厂货号：11846S
• 产品分类：抗体 > 一抗 > 其它类型一抗
• 应用分类：

描述：

用来检测具有HA表位标签的外源表达蛋白。用来检测具有HA表位标签的外源表达蛋白。CST的抗体与结合甲酰基芳酰胺磁珠相互结合被固化。HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate)可用于HA标签的重组蛋白的免疫共沉淀分析。免疫沉淀分析COS-7细胞中HA标签的Akt，所用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control (Magnetic Bead Conjugate) #8726 (泳道2) 或 HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) (泳道3)。泳道1为10%的细胞提取物。采用HA-Tag (6E2) Mouse mAb (HRP Conjugate) #2999进行western分析。

在免疫印迹、免疫沉淀和免疫染色等技术中，表位标签用于标记和检测蛋白。由于它们的体积很小，它们一般不会影响标记蛋白的生化特性。HA标签式从流感血凝素蛋白的一个表位演化而来的。已经作为常规标签被广泛应用于表达载体中（1）。

1. Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.

原厂资料：

Specificity / Sensitivity

 HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) detects exogenously expressed proteins containing the HA epitope tag.

Specificity / Sensitivity

 HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) detects exogenously expressed proteins containing the HA epitope tag.

Description

 This Cell Signaling Technology antibody is immobilized by the covalent reaction of formylbenzamide-modified antibody with hydrazide-activated magnetic bead. HA-Tag (C29F4) Rabbit mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation assays of HA-tagged recombinant proteins.

Directions for Use

 Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution using our 6-Tube Magnetic Separation Rack #7017. Place the tubes containing the beads in the Magnetic Separation Rack and wait 1 to 2 minutes for the solution to clear before carefully removing the supernatant. Remove the tubes from the Magnetic Separation Rack, add new solution and resuspend the beads by gently vortexing or rocking the tube.

Background

 Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

Background

 Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.

1. Field, J. et al. (1988) Mol Cell Biol 8, 2159-65.

注意事项：

Storage: Supplied in PBS Buffer (pH 7.2), 0.1% Tween®20. Store at 4°C. Do not aliquot the antibodies.

Directions for Use: Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml in 1X Cell Lysis Buffer (10X) #9803. See protocol for more details.

Recommended Antibody Dilutions: Immunoprecipitation 1:20

For product specific protocols please see the web page for this product at www.cellsignal.com.