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Cell Fractionation Antibody Sampler Kit

Cell Fractionation Antibody Sampler Kit

产品编号：CST-11843S 品牌：Cell Signaling Technology 原厂货号：11843S
产品分类：抗体 > 一抗 > 复合抗体试剂盒
应用分类：

包装：

1Kit

运保温度:

-20°C

到货周期：

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其它组分：

Histone H3 (D1H2) XP® Rabbit mAb【子货号： 4499，包装：40 µl，，运保温度：-20°C】

AIF (D39D2) XP® Rabbit mAb 【子货号：5318，包装：40 µl，，运保温度：-20°C】

Vimentin (D21H3) XP® Rabbit mAb 【子货号：5741，包装：40 µl，，运保温度：-20°C】

Anti-rabbit IgG, HRP-linked Antibody【子货号：7074，包装：40 µl，，运保温度：-20°C】

MEK1/2 (D1A5) Rabbit mAb 【子货号：8727，包装：40 µl，，运保温度：-20°C】

描述：

Cell Fractionation Antibody Sampler Kit中的每种抗体都能识别内源性水平的它们各自的目标蛋白。抗体与其他家族成员没有交叉反应。每种抗体都用Cell Fractionation Kit #9038进行了验证。在不同细胞和组织中，这些蛋白的表达可能有所不同。兔单克隆抗体通过用合成肽免疫动物制备，该合成肽分别是人AIF蛋白Ala520周围的残基、人组蛋白H3 蛋白的羧基端、人MEK1蛋白Ala220周围的残基和人波形蛋白Arg45周围的残基。兔单克隆抗体通过用合成肽免疫动物制备，该合成肽分别是人AIF蛋白Ala520周围的残基、人组蛋白H3 蛋白的羧基端、人MEK1蛋白Ala220周围的残基和人波形蛋白Arg45周围的残基。

蛋白质的亚细胞定位知识可能揭示其潜在的在多种细胞过程中的作用。Cell Fractionation Antibody Sampler Kit中的抗体可以作为一个标示物，确保每一个亚细胞成分都与下一个成分有效分离了。MEK1和MEK2，也被称为MAPK或Erk激酶，是双特异性蛋白激酶，在分裂原活化蛋白激酶级联反应中起作用（1）。凋亡诱导因子（AIF）是一种广泛表达的黄素蛋白，在不依赖半胱天冬酶的细胞凋亡中有重要作用（2）。核心组蛋白，H3是组成核小体的染色质的主要构件之一（3）。波形蛋白是一种间充质来源的细胞专一的中间纤维，它有助于细胞骨架的形成（4）。MEK1/2，AIF，组蛋白H3和波形蛋白分别位于细胞质，线粒体，细胞核和细胞骨架。

原厂资料：

Specificity / Sensitivity

Each antibody in the Cell Fractionation Antibody Sampler Kit recognizes endogenous levels of its respective target protein. The antibodies do not cross-react with other family members.   Each antibody has been validated using the Cell Fractionation Kit #9038. Expression of these proteins may vary in different cells and tissues.

Source / Purification

Rabbit monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala520 of human AIF protein, the carboxy terminus of human histone H3 protein, residues surrounding Ala220 of human MEK1 protein, and residues surrounding Arg45 of human vimentin protein.

Description

The Cell Fractionation Antibody Sampler Kit provides an economical means for determining the purity of each distinctly separated subcellular fraction by western blot using Cell Signaling Technology's Cell Fractionation Kit #9038. This antibody sampler kit includes enough primary antibody to perform at least four western blots per primary antibody.

Background

Knowledge of the subcellular location of a protein may reveal the potential role it plays in a variety of cellular processes. Antibodies in the Cell Fractionation Antibody Sampler Kit can be used as a marker to ensure that each subcellular fraction is efficiently separated from the next. MEK1 and MEK2, also called MAPK or Erk Kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade (1). Apoptosis-inducing factor (AIF) is a ubiquitously expressed flavoprotein that plays a critical role in caspase-independent apoptosis (2). Core histone protein, H3 is one of the primary building blocks of chromatin that makes up the nucleosome (3). Vimentin is a cell-specific intermediate filament with mesenchyme origin that contributes to making up of the cytoskeleton (4). MEK1/2, AIF, histone H3, and vimentin localize to the cytoplasm, mitochondria, nucleus, and cytoskeleton, respectively.

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

*Species cross-reactivity is determined by western blot.

** Anti-rabbit secondary antibodies must be used to detect this antibody.

Recommended Antibody Dilutions:

Western blotting 1:1000