Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb

• 产品编号：CST-11817S      品牌：CST       原厂货号：11817S
• 产品分类：抗体 > 一抗 > 磷酸化抗体
• 应用分类：

描述：

Phospho-GRB10 (Ser476) (D4E6)mAb兔单抗可以识别Ser476被磷酸化的内源性GRB10蛋白。

单克隆抗体由合成肽段免疫动物产生，该肽段与人GRB10的Ser476邻近残基序列一致。

单克隆抗体由合成肽段免疫动物产生，该肽段与人GRB10的Ser476邻近残基序列一致。

近期，GRB10被认为是mTORC1的直接底物（5）。胰岛素刺激后，mTORC1可以磷酸化GRB10的Ser150, Ser428, 和Ser476（5). GRB7接头蛋白家族由GRB7, GRB10和GRB14组成，都含有一个氨基末端富含脯氨酸SH3结合结构域，后面紧随着PH, PBS, 和 SH2结构域。每个家族成员都有多个剪切异构体（1）。有报道表明GRB10可以和多种受体酪氨酸激酶（RTKs）以及下游信号分子包括Raf，Akt和Nedd4结合（1,2）。尽管最初认为GRB10只能在丝氨酸残基处磷酸化（3），Src激酶家族成员可以磷酸化GRB10的Tyr67（4）。

原厂资料：

Applications Key: W=Western Blotting, IP=Immunoprecipitation

Homology

Species predicted to react based on 100% sequence homology: Rat

Specificity / Sensitivity

Phospho-GRB10 (Ser476) (D4E6) Rabbit mAb recognizes endogenous levels of GRB10 protein only when phosphorylated at Ser476.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser476 of human GRB10 protein.

Background

Recently GRB10 was shown to be a direct substrate of mTORC1 (5). It is phosphorylated by mTORC1 at Ser150, Ser428, and Ser476 upon insulin stimulation (5).The GRB7 family of adaptor proteins consist of GRB7, GRB10 and GRB14, which all contain an amino-terminal proline-rich SH3 binding domain, followed by PH, PBS, and SH2 domains. Each member of the family has several splice variants (1). It has been reported that GRB10 interacts with many receptor tyrosine kinases (RTKs) as well as downstream signal molecules including Raf, Akt, and Nedd4 (1,2). Although it was originally thought that GRB10 is exclusively phosphorylated at serine residues (3), Src kinase family members have been shown to phosphorylate GRB10 at Tyr67 (4).

注意事项：

Storage: Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
*Species cross-reactivity is determined by western blot.
** Anti-rabbit secondary antibodies must be used to detect this antibody.
Recommended Antibody Dilutions:
Western Blotting 1:1000
Immunoprecipitation 1:50