Description: This FlowCytomix Simplex Kit is designed for the measurement of Human CD44var in an immunoassay analyzed on a flow cytometer. Together with the FlowCytomix Human Basic Kit (cat. BMS8420FF), this kit can be used to detect CD44var alone or can be multiplexed with other Simplex Kits to measure a variety of analytes.
This kit contains bead population A7.
Alternative splicing of the mRNA gives rise to the different isoforms, containing inserts of varying sizes in the extracellular domain of the molecule (exons v2-v10). All CD44 isoforms are variably glycosylated. In contrast to standard CD44 (CD44std) which is almost ubiquitously expressed, the variety of CD44 isoforms (CD44var) have a much more restricted distribution, e.g., on activated lymphocytes and macrophages (exon v6).
The sCD44var(v6) ELISA detects all circulating CD44 isoforms comprising the sCD44var(v6) sequences.
Determination of sCD44var(v6) will provide more detailed insight into different pathological modifications during cancer and other diseases.
Components
1 vial (175 ul) Fluorescent Beads (20x) coated with monoclonal antibody to human CD44var (v6), Bead Population A7
2 vials human CD44var (v6) Standard (lyophilized): 2 ug/ml upon reconstitution
1 vial (350 ul) Biotin-Conjugate (20x) anti-human CD44var (v6) monoclonal antibody
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