Description: The mouse IL-1alpha ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse IL-1alpha. The mouse IL-1alpha ELISA is for research use only. Not for diagnostic or therapeutic procedures.
The interleukin-1 species represent an important family of biologically active mono nuclear cell-derived proteins which are involved in inflammatory reactions and in immune responses. Two distinct IL-1 species, IL-1alpha and IL-1beta, have been identified. They share similarities such as the same molecular weight, similar biological effects and the same receptors on target cells. IL-1 proteins are produced by macrophages, monocytes and various other cell types such as adult T cell leukemias, fibroblasts, epithelial or endothelial cells, neutrophils and astrocytes. Their biological properties include pyrogenicity, bone resorption, presentation of antigen to T cells and stimulation of B and T lymphocyte proliferation.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse IL-1alpha
Biotin-Conjugate anti-mouse IL-1alpha monoclonal antibody
Streptavidin-HRP
Mouse IL-1alpha Standard lyophilized, 1000 pg/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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