Description: The human sCD28 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human sCD28. The human sCD28 ELISA is for research use only. Not for diagnostic or therapeutic procedures.
CD28 is a 44 kDa, disulphide- bonded, homodimeric glycoprotein, which is constitutively expressed on the surface of the majority of T-cells. CD28 is structurally very closely related to another molecule expressed in activated T-cells, CTLA-4. Ligation of CD28 by its counter receptor, B7, expressed on the surface of antigen presenting cells, has been shown to induce signals that, in synergy with those derived from engagement of the T-cell receptor by an antigen bound to a major histocompatibilty complex, enhance proliferation and cytokine production. Manipulation of this interaction can have dramatic effects on the outcome of T-cell activation. Considerable research has been done on the CD28/B7 costimulatory pathway. Blocking thereof results in immunosuppression with implications for the treatment of autoimmune diseases, allergy, organ transplantation and graft versus host disease.
Activating the CD28/B7 pathway could be useful for including the immune system to recognize and eliminate tumors that evade the immune system.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human sCD28
Biotin-Conjugate anti-human sCD28 monoclonal antibody
Streptavidin-HRP
Human sCD28 Standard lyophilized, 100 ng/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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