Description: The human Cathepsin L ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human Cathepsin L. The human Cathepsin L ELISA is for research use only. Not for diagnostic or therapeutic procedures.
Cathepsin L is closely related to the recently described cathepsin V (L2) and the sequence indentity of almost 80% between them requires antibodies for the detection of cathepsin L which do not crossreact with cathepsin V. This requirement can only be met by epitope specific monoclonal antibodies as are used in BMS257.
Cathepsin L is a lysosomal cysteine proteinase consisting of a heavy chain of about 25 kDa and a light chain of about 5 kDa derived proteolytically from the same precursor. Cathepsin L has been shown to be produced by many different cell types. Since cathepsin L is capable of degrading protein constituents of the extracellular matrix it is assumed to play a crucial role in tumor progression and metastasis and a number of other disorders where the destruction of the extracellular matrix is the major cause of disease (e.g. rheumatoid arthritis, neurodegeneration).
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human Cathepsin L
Biotin-Conjugate anti-human Cathepsin L monoclonal antibody
Streptavidin-HRP
Human Cathepsin L Standard lyophilized, 100 ng/ml upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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