Description: The human IFN-gamma ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IFN-gamma. The human IFN-gamma ELISA is for research use only. Not for diagnostic or therapeutic procedures.
IFN-gamma, also called Type II interferon, is a homodimeric glycoprotein containing approximately 21 to 24 kD subunits. The human IFN-gamma gene, situated on chromosome 12, contains three introns; the four exons code for a polypeptide of 166 amino acids, 20 of which constitute the signal peptide. In contrast to IFN-alpha and IFN-beta synthesis, which can occur in any cell, production of IFN-gamma is a function of T cells and NK cells.
IFN-gamma has antiviral and antiparasitic activities and also inhibits the proliferation of a number of normal and transformed cells. IFN-gamma synergises with TNF-alpha and TNF-beta in inhibiting the proliferation of various cell types.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IFN-gamma
Biotin-Conjugate anti-human IFN-gamma monoclonal antibody
Streptavidin-HRP
Human IFN-gamma Standard lyophilized, 200 ng/mL upon reconstitution
Sample Diluent
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Red-Dye
Adhesive Films
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