Description: The human IgA ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IgA. The human IgA ELISA is for research use only. Not for diagnostic or therapeutic procedures.
IgA comprises approximately 15% of all immunoglobulins in healthy human serum. IgA in serum is mainly monomeric, but in secretions, such as saliva, tears, colostrums, mucus, sweat, gastric fluid, IgA is found as a dimer where they are connected by a joining peptide. Most IgA is present in secreted form. This is believed to be due to ist properties in preventing invading pathogens by attaching and penetrating epithelial surfaces. IgA is just a very weak complement activating antibody; hence it does not induce bacterial cell lysis via the complement system. However secretory IgA works together with lysozymes, also present in many secreted fluids, which hydrolyze carbohydrates in bacterial cell walls enabling the immune system to clear the infection. IgA is predominantly found on epithelial cell surfaces where it acts as a neutralizing antibody.
Components
Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human IgA
HRP-Conjugate anti-human IgA monoclonal antibody
human IgA Standard lyophilized, 200 ng/mL upon reconstitution
Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
Substrate Solution (tetramethyl-benzidine)
Stop Solution (1M Phosphoric acid)
Blue-Dye
Green-Dye
Adhesive Films